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目的:探讨冬虫夏草对T淋巴细胞亚群及小肠黏膜屏障免疫功能的影响。方法:将大鼠分为对照组和实验组。实验组大鼠给予冬虫夏草5g/(kg·d)灌胃;对照组给予等量的等渗盐水灌胃,两组均自由进食、饮水。于灌胃第4、7和10天分别抽取8只大鼠的血,用流式细胞仪检测外周血液中CD3+、CD4+、CD8+的百分率,计算CD4+/CD8+比例。同时取大鼠小肠黏膜,行常规病理计数上皮内淋巴细胞(IEL)的比例,用免疫组化染色检测CD4+、CD8+,计算CD4+/CD8+的比例。结果:实验组大鼠第4天外周血中CD4+淋巴细胞即有明显提高,CD4+/CD8+的比例明显升高,至第7和第10天变化更为明显。肠黏膜上皮内淋巴细胞的数量和CD4+/CD8+比例的变化也较明显,差异有显著性意义(P<0.05)。结论:冬虫夏草可使正常大鼠外周血T淋巴细胞含量增加,同时可增强小肠黏膜的免疫屏障功能。
Objective: To investigate the effect of Cordyceps sinensis on T lymphocyte subsets and intestinal mucosal barrier immune function. Methods: Rats were divided into control and experimental groups. Rats in the experimental group were given intragastric administration of Cordyceps sinensis 5g/(kg·d); the control group was given equal volume of isotonic saline to gavage; both groups were allowed to eat and drink freely. Blood samples from 8 rats were collected on the 4th, 7th, and 10th day of intragastric administration. Percentages of CD3+, CD4+, and CD8+ in peripheral blood were measured by flow cytometry, and CD4+/CD8+ ratios were calculated. At the same time, the rat intestinal mucosa was removed, and the proportion of intraepithelial lymphocytes (IEL) was counted by routine pathology. CD4+ and CD8+ were detected by immunohistochemical staining, and the proportion of CD4+/CD8+ was calculated. RESULTS: In the experimental group, CD4+ lymphocytes in the peripheral blood of the experimental group were significantly increased, and the proportion of CD4+/CD8+ was significantly increased, and the change was more pronounced on the 7th and 10th days. The changes in the number of intestinal epithelial lymphocytes and the ratio of CD4+/CD8+ were also significant, with significant differences (P<0.05). Conclusion: Cordyceps can increase the content of T lymphocytes in peripheral blood of normal rats, and it can also enhance the immune barrier function of intestinal mucosa.