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致病性大肠杆菌藉产生定居因子抗原肠毒素而致人类腹泻。业已证明:定居因子抗原I(CFA/1)由质粒指令产生,分子量为60×10~6,丧失此质粒的菌株同时丧失产生热稳定性肠毒素(ST)的能力,由于已鉴定过的所有CFA/I~+菌株同时也是ST~+菌株,提示CFA/I与ST之间似有密切关系,近已见有CFA/I—ST质粒从O78菌株传递至E.ColiK-12菌株之类的报道。本文作者检查了六株O78菌株和一株O63:H-菌株以确定CFA/I—ST质粒是否常见并比较了所检出质粒的特性。作者采用乳鼠试验检测ST,采用甘露糖抗性血凝试验及免疫扩散试验以检测CFA/I分别采用μ_2噬菌体及一些大肠杆菌噬菌体来
Pathogenic Escherichia coli causes human diarrhea by producing the colonizing factor antigen enterotoxin. It has been demonstrated that the Settle Factor Antigen I (CFA / 1) is produced by a plasmid instruction with a molecular weight of 60 x 10 ~ 6 and that the loss of this plasmid simultaneously loses its ability to produce a thermostable enterotoxin (ST) CFA / I ~ + strain is also ST ~ + strain, suggesting that there is a close relationship between CFA / I and ST. Nearly CFA / I-ST plasmid has been transferred from O78 strain to E.coli K-12 strain Reported. The authors examined six strains of O78 and one strain of O63: H-H to determine if CFA / I-ST plasmids were common and to compare the properties of the plasmids detected. The authors used the suckling rat test to detect ST, using the mannose-resistant hemagglutination test and the immunodiffusion test to detect the CFA / I respectively using the phage 2-2 and some E.coli phage