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目的:建立中药淡竹叶中日本当药黄素的RP-HPLC含量测定方法。方法:高效液相色谱法,采用Cosmosil 5 C18-ms-Ⅱ色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.05%醋酸水溶液(14:86),流速1.0 mL·min-1,分析时间20 min,检测波长350 nm,进样体积10μL。结果:日本当药黄素进样量与峰面积呈良好线性关系,线性范围为0.3～5.0μg,r=1.000 0(n=5),回收率为100.2%(n=6)。结论:该方法首次测定了8个地区淡竹叶药材中日本当药黄素的含量,该方法准确、简便、重复性好。
OBJECTIVE: To establish a RP-HPLC method for the determination of radix beet in Japanese traditional medicine. METHODS: High performance liquid chromatography (HPLC) was performed on a Cosmosil 5 C18-MS-Ⅱ column (4.6 mm × 250 mm, 5 μm) with a mobile phase of acetonitrile-0.05% acetic acid aqueous solution of 14:86 at a flow rate of 1.0 mL · min- Analysis time 20 min, detection wavelength 350 nm, injection volume 10 μL. Results: There was a good linear relationship between the injection amount of flavin and the peak area in Japan. The linear range was 0.3-5.0 μg, r = 1.000 0 (n = 5), and the recovery was 100.2% (n = 6). CONCLUSION: This method is the first time to determine the content of flavin in Japanese bamboo in eight areas. The method is accurate, simple and reproducible.