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以鲫鱼的血液、肌肉、肝、肾为材料,采用苯酚-氯仿法、试剂盒法提取鲫鱼基因组DNA,对不同方法提取的结果进行了紫外分光光度、琼脂糖凝胶电泳、微卫星PCR扩增等方法的鉴定。结果表明不同方法提取的鲫鱼基因组DNA浓度在385.8～3167.5ng/μL,A260/280比值处于1.66～1.87,所提取的DNA适合微卫星PCR扩增。因此,用苯酚-氯仿法对鲫鱼的血液、肌肉、肝、肾,用试剂盒法对鲫鱼的血液均能提取鲫鱼基因组DNA。
The genomic DNA of Carassius auratus was extracted with phenol-chloroform method and kit method by using the blood, muscle, liver and kidney of crucian carp. The results of different methods were analyzed by ultraviolet spectrophotometry, agarose gel electrophoresis and microsatellite PCR Identification of other methods. The results showed that the genomic DNA of Carassius auratus extracted by different methods was in the range of 385.8-3167.5 ng / μL, the A260 / 280 ratio was in the range of 1.66-1.87, and the extracted DNA was suitable for microsatellite DNA amplification. Therefore, using the method of phenol-chloroform to test the blood, muscle, liver and kidney of Carassius auratus, the genomic DNA of Carassius auratus can be extracted from the blood of the carp using the kit method.