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目的 为人神经干细胞的基础研究及临床应用提供细胞模型。 方法 采用无血清培养技术 ,分离培养了 10～ 14周人胚大脑皮层细胞 ,并用神经上皮干细胞蛋白 (Nestin)免疫组化鉴定培养细胞 ;5 %胎牛血清诱导其分化 ,神经丝 2 0 0 (NF 2 0 0 )和胶质纤维酸性蛋白 (GFAP)免疫组化鉴定分化细胞。 结果 获得了大量未分化、呈簇状悬浮生长的神经干细胞团 ,且能被诱导分化成神经元和神经胶质细胞。经传 12代后仍具干细胞特性。 结论 本实验成功地分离培养了人胚胎大脑皮层神经干细胞 ,为神经干细胞的基础研究和临床应用奠定了基础。
Objective To provide a cell model for basic research and clinical application of human neural stem cells. Methods Cultured human embryonic cerebral cortex cells were isolated and cultured in serum-free medium for 10-14 weeks. The cultured cells were identified by Nestin immunohistochemistry. Differentiation was induced by 5% fetal bovine serum. NF 2 0 0) and glial fibrillary acidic protein (GFAP) immunohistochemical identification of differentiated cells. The results obtained a large number of undifferentiated, cluster-like growth of neural stem cell clusters, and can be induced to differentiate into neurons and glial cells. After 12 passages, it still has stem cell characteristics. Conclusion The experiment successfully isolated and cultured human embryonic cerebral cortex neural stem cells, and laid the foundation for the basic research and clinical application of neural stem cells.