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目的:观察丹参对三氯化铝(Alcl_3)所致的阿尔兹海默病(AD)模型小鼠学习记忆能力的影响及其脑组织和血清中乙酰胆碱酯酶(AchE)含量的变化。方法:AD模型组:给小鼠每日按400mg/kg灌胃4%的AlCl_3溶液,连续灌胃60d。正常对照组:每日灌胃与AlCl_3等同剂量的生理盐水。丹参治疗组:每日灌胃AlCl~(3+)丹参溶液1mL。丹参对照组:每日只灌胃丹参溶液1mL。实验周期为60d。给药结束后进行Morris水迷宫训练,24h后进行学习记忆功能测试和乙酰胆碱酯酶含量的测定。结果:丹参治疗组、丹参对照组与正常对照组比较,小鼠的逃避潜伏期以及其脑组织和血清中的乙酰胆碱酯酶含量无明显变化,差异无统计学意义(P>0.05)。与模型组小鼠比较,丹参治疗组小鼠的逃避潜伏期明显缩短(P<0.05),并且其脑组织和血清中的乙酰胆碱酯酶含量比模型组明显降低(P<0.05)。结论:丹参能有效降低AlCl_3致AD模型小鼠脑内及血清中胆碱酯酶活性,明显改善AD模型小鼠的学习记忆功能,这可能是丹参可防治阿尔兹海默病的机制之一。
Objective: To observe the effect of Salvia miltiorrhiza on the learning and memory abilities of Alzheimer’s disease (AD) model mice induced by aluminum chloride (AlCl 3) and the changes of acetylcholinesterase (AchE) content in brain tissue and serum. Methods: AD model group: The mice were treated with 400 mg / kg 4% AlCl 3 daily for 60 days. Normal control group: daily oral administration of AlCl_3 equivalent dose of saline. Salvia treatment group: daily gastric perfusion AlCl ~ (3 +) Salvia solution 1mL. Salvia control group: a day only gavage Salvia solution 1mL. The experiment period is 60d. Morris water maze training was performed after the administration, and learning and memory function tests and determination of acetylcholinesterase levels were performed after 24 hours. Results: Compared with the normal control group, the escape latency and the content of acetylcholinesterase in brain tissues and serum of Salvia miltiorrhiza group and Salvia miltiorrhiza group had no significant difference (P> 0.05). Compared with the model group, the escape latency of the mice in the Salvia miltiorrhiza treatment group was significantly shorter (P <0.05), and the content of acetylcholinesterase in the brain tissue and serum was significantly lower than the model group (P <0.05). Conclusion: Salvia miltiorrhiza can effectively reduce the activity of cholinesterase in brain and serum of Alzheimer disease mice induced by AlCl 3 and improve the learning and memory abilities of Alzheimer ’s disease mice. This may be one of the mechanisms of Salvia miltiorrhiza in preventing Alzheimer’ s disease.