Dual oxidase(duox)-derived reactive oxygen species(ROS)have been correlated with neuronal polarity,cerebellar development,and neuroplasticity.However,there have not been many comprehensive studies of the effect of individual duox isoforms on central-axon regen-eration in vivo.Here,we explored this question in zebrafish,an excellent model organism for central-axon regeneration studies.In our research,mutation of the duox gene with CRISPR/Cas9 significantly retarded the single-axon regeneration of the zebrafish Mauthner cell in vivo.Using deep transcriptome sequencing,we found that the expression levels of related functional enzymes in mito-chondria were down-regulated in duox mutant fish.In vivo imaging showed that duox mutants had significantly disrupted mitochondrial transport and redox state in single Mauthner-cell axon.Our research data provide insights into how duox is involved in central-axon regeneration by changing mitochondrial transport.