论文部分内容阅读
目的观察人神经干细胞(NSCs)移植治疗脑性瘫痪(CP)并重度视觉障碍患儿的临床疗效,探讨重度视觉障碍患儿可能的治疗方法。方法11例严重视觉障碍的CP患儿行人NSCs移植。取孕11周正常发育人胚胎前脑细胞,在培养基中进行培养,培养基中添加表皮生长因子20μg/L、碱性成纤维细胞生长因子10μg/L、白血病抑制因子1μg/L,培养至第14天,获取生长旺盛的NSCs小集落,制成细胞悬液,并经前囟穿刺注入患儿右侧侧脑室。术后即进行临床视觉功能的观察,同时第28天进行闪光视觉诱发电位(F-VEP)、功能核磁(fMRI)及眼底复查,术后视觉检查追踪至6个月。结果移植后7例患儿在术后第6-13天即出现视觉功能改善,表现为临床视物及视物追踪的明显好转。同时伴F-VEP、fMRI的改变。另4例患儿无效。无1例患儿出现病情的倒退及其他不良并发症。结论人NSCs移植能改善部分CP并重度视觉障碍患儿的视觉功能。
Objective To observe the clinical efficacy of transplantation of human neural stem cells (NSCs) in children with cerebral palsy (CP) and severe visual impairment and to explore the possible treatment of severe visual impairment in children. Methods 11 cases of severe visual impairment in pediatric NSCs transplantation. At 11 weeks of gestation, normal human embryonic forebrain cells were cultured and cultured in culture medium. EGF was supplemented with 20μg / L epidermal growth factor, 10μg / L basic fibroblast growth factor and 1μg / L leukemia inhibitory factor. On day 14, small colonies of well-growing NSCs were obtained, and the cells were suspended in suspension and infused into the right lateral ventricle through the anterior fontanel. Clinical visual function was observed after operation, and flash visual evoked potential (F-VEP), functional magnetic resonance imaging (fMRI) and fundus examination were performed on the 28th day. Postoperative visual inspection was performed to 6 months. Results 7 cases of children after transplantation showed visual function improvement 6-13 days after operation, which manifested as obvious improvement of clinical visual material and visual material tracing. At the same time with the F-VEP, fMRI changes. The other 4 cases were invalid. None of the children had a regression of their condition and other adverse complications. Conclusion Transplantation of human NSCs can improve the visual function of children with partial CP and severe visual impairment.