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目的:研究中药复方“松友饮”含药血清对体外培养的人肝癌MHCC97H细胞增殖、侵袭潜能的影响。方法:以MHCC97H细胞构建裸鼠原位移植瘤模型,84只成模裸鼠随机平均分成对照组和“松友饮”组,肝脏接种肿瘤24 h内开始1次/天灌胃给药。在第1、4、7、14、21、28、35次灌胃结束后2h,每组分别处死6只裸鼠收集血样。应用含药血清体外干预MHCC97H细胞,检测细胞增殖、侵袭潜能变化。结果:“松友饮”灌胃给药1、4、7天的荷HCC裸鼠含药血清体外抑制HCC作用逐渐增强,给药7、14、21、28、35天的含药血清抑制作用无统计学差异。灌胃7天的“松友饮”含药血清抑制HCC增殖的作用与中剂量卡培他滨作用近似,抑瘤率分别为37.4%、41.1%。给药7天的含药血清抑制HCC侵袭潜能达到最强,“松友饮”组穿膜细胞数明显少于对照组,为26.0±3.6和37.3±2.8。结论:给药7天的含药血清达到稳态血药浓度,对MHCC97H细胞的体外抑制作用达到最强。
Objective: To study the effect of Chinese herbal compound “Songyouyin” containing serum on proliferation and invasion potential of human hepatoma MHCC97H cells cultured in vitro. Methods: The in situ xenograft model of nude mice was established by MHCC97H cells. Eighty-four nude mice were randomly divided into control group and “Songyouyin” group, and the liver was inoculated intragastrically once daily for 24 hours. On the 1st, 4th, 7th, 14th, 21st, 28th and 35th days after the end of gavage, 2 nude mice were sacrificed and blood samples were collected. In vitro drug-containing serum intervention MHCC97H cells, detection of cell proliferation, invasion potential changes. Results: “Song Youyin” intragastric administration of 1,4,7 days of HCC nude mice serum containing HCC inhibitory effect in vitro gradually increased, administered 7,14,21,28,35 days of drug-containing serum inhibition No significant difference in effect. The effect of 7-day “Songyouyin” containing serum on HCC proliferation was similar to that of medium dose capecitabine. The tumor inhibition rates were 37.4% and 41.1% respectively. The drug-containing serum administered for 7 days achieved the strongest HCC invasive potential, and the number of transmembrane cells in the “Songyouyin” group was significantly less than that of the control group (26.0 ± 3.6 and 37.3 ± 2.8). Conclusion: The drug-containing serum administered for 7 days reaches steady-state plasma concentration, and has the strongest inhibitory effect on MHCC97H cells in vitro.