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菠菜（ＳｐｉｎａｃｉａｏｌｅｒａｃｅａＭｉｌ．）品种“华菠一号”生长在室内条件下，以０．０和１３．０ｋＪ·ｍ－２·ｄ－１的ＵＶＢ进行照射处理，研究了ＵＶＢ对菠菜叶片内类黄酮和脂质过氧化作用的影响。结果表明：ＵＶＢ辐射降低了菠菜的单株叶鲜重，诱导了体内吸收ＵＶＢ的类黄酮化合物的大量积累，并使可溶性蛋白含量与叶绿素含量下降。ＵＶＢ辐射能使菠菜叶片内Ｏ－·２及丙二醛（ＭＤＡ）含量大幅度上升，而使抗坏血酸（ＡｓＡ）含量下降。ＵＶＢ辐射能使菠菜叶片内过氧化物酶（ＰＯＸ）、ＳＯＤ的活性大幅度上升，而过氧化氢酶（ＣＡＴ）活性仅略有增加。初步推测：ＵＶＢ辐射首先诱导菠菜叶片内Ｏ－·２的积累，并导致膜脂质过氧化作用，从而抑制菠菜生长。Ｏ－·２的积累能诱导抗氧化酶活性的上升，但抗氧化酶活性的上升和吸收ＵＶ的类黄酮化合物的积累并不能扭转ＵＶＢ对菠菜的伤害。
Spinachia spinach (Spinacia oleraceaMil.) Variety “Hua-spinach” was grown in indoor conditions, with 0.0 and 13.0kJ · m-2 · d-1 of UV B irradiation treatment UVB on the spinach leaves Effect of Flavonoids and Lipid Peroxidation in. The results showed that: UVB radiation decreased the fresh weight per plant of spinach, induced a large accumulation of flavonoid compounds absorbing UVB in vivo, and decreased the content of soluble protein and chlorophyll. UV B radiation in spinach leaves O- · 2 and malondialdehyde (MDA) content increased significantly, leaving ascorbic acid (AsA) decreased. UV B radiation in leaves of spinach leaves peroxidase (POX), SOD activity increased significantly, and catalase (CAT) activity increased only slightly. Preliminary speculation: UV B radiation first induced the accumulation of O- 2 in spinach leaves, and lead to membrane lipid peroxidation, thereby inhibiting spinach growth. The accumulation of O-2 could induce the increase of antioxidant enzyme activity, but the increase of antioxidant enzyme activity and the absorption of UV-flavonoids did not reverse the damage of UV-B to spinach.