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目的：建立测定人血浆丙泊酚（异丙酚） 浓度的ＨＰＬＣ 方法，为临床异丙酚的药理学研究提供科学的分析技术。方法：用环己烷提取血浆中的异丙酚，用ＨＰＬＣ测定异丙酚含量。测定异丙酚以μＢｏｎｄａｐａｋ Ｃ１８ 柱为分离柱，乙腈水（５５∶４５）为流动相，以百里酚为内标物，用紫外检测，波长２７０ ｎｍ 。结果：本方法回收率高，重现性好，线性范围为０－１ ～４－０ μｇ·ｍｌ－ １ ，最低检测浓度为０－０５ μｇ·ｍｌ－ １ 。结论：本方法灵敏、快速、选择性强，具有较高的精密度和准确度，适用于临床血药浓度检测。
OBJECTIVE: To establish a HPLC method for the determination of propofol in human plasma and to provide a scientific analytical technique for the pharmacological study of propofol in clinical practice. Methods: Propofol in plasma was extracted with cyclohexane and propofol content was determined by HPLC. Determination of propofol to μ Bondapak C18 column as the separation column, acetonitrile water (55:45) as the mobile phase to thymol as an internal standard, with UV detection wavelength of 270 nm. Results: The method has high recovery and good reproducibility. The linear range is 0-1 ~ 4-0 μg · ml-1, and the lowest detection concentration is 0-05 μg · ml-1. Conclusion: The method is sensitive, rapid, selective, with high precision and accuracy, suitable for clinical blood concentration detection.