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用赖型０１７株钩体ＤＮＡ基因库中筛选出的重组克隆ｐＣＸ７制备重组质粒，再以￣（３２）Ｐ标记为探针，对１１个血清群的１８株问号钩体、双曲钩体ＰａｔｏｃＩ株和细螺旋体ｉｌｌｉｎｉ３０５５株ＤＮＡＳｏｕｔｈ－ｅｒｎ杂交。放射自显影结果显示：双曲钩体ＰａｔｏｃＩ株和细螺旋体ｉｌｌｉｎｉ３０５５株ＤＮＡ未获杂交阳性区带；ｊａｖａｎｉｃａ（爪哇）、ｍａｎｈａｏ２（曼耗２）和ｒａｎａｒｕｍ（蛙）三个低毒力血清型的问号状钩体ＤＮＡ亦为阴性；１５株问号状钩体ＤＮＡ均出现了杂交信号，而且不同群型钩体ＤＮＡ杂交带型明显不同，赖型钩体０１７株（强毒株）与６０１株（弱毒株）杂交带型也有细微差别。作者认为，以ｐＣＸ７探针进行Ｓｏｕｔｈｅｒｎ杂交可望作为钩体分类和鉴定的工具或参考。
The recombinant plasmid pCX7 was screened from the 017 leptospira DNA library of Lai gene and the recombinant plasmids were prepared by using the (32) P-labeled probe. The 18 serogroups of 18 serogroups, The strain was hybridized with DNASouth-ern of the illini3055 strain. The results of autoradiography showed that there was no hybridization positive band between PatocI and illini3055 DNA of the leptospira; javanica (Java), manhao2 (manx2) and ranarum (frog) three low virulent serotypes The results showed that there was a hybridization signal in the DNA of all the 15 tick-like leptospira species, and the banding patterns of DNA in different groups of leptospira were significantly different. The 017 isolates (virulent strains) and 601 isolates Strains also have subtle differences. The authors believe that Southern hybridization with the pCX7 probe is expected as a tool or reference for the classification and identification of leptospira.