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本文报道柔红霉素产生菌——天兰淡红链霉菌原生质体的制备和再生方法,并从再生菌落中筛选得到摇瓶发酵单位提高5%的高产菌株30#。将再生高产株经铜蒸气激光辐照,得到在生产罐中发酵水平比对照出发菌株提高12.9%的高产菌株82#。
In this paper, we reported the preparation and regeneration of protoplasts of daunomycin-producing Streptomyces daunomycinum, and screening high-yield strain 30 # with 5% increase of fermentation units in shake flasks from regenerated colonies. High-yield regenerated plants were irradiated with copper vapor laser to obtain high-yielding strain 82 # with a 12.9% higher fermentation level than the control strain in the production tank.