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建立了以RP2（25～40μm）为预处理柱，水为预处理流动相，以0．2mol／L乙酸和0．2mol／L乙酸按为净化清洗液，血浆直接进样，在线（on-line）固相浓缩净化样品；以ShimpackCLC－ODS为分析柱（15cm×6mmID），甲醇-乙酸按（0.2mol／L，pH2.7）（14：86）为分析流动相，267um波长检测，外标法定量测定人体血浆中法莫替丁的浓度的方法．该法切换系统构成简单，仪需要一个切换阀和两个溶剂系统。200μl血浆仅以。05mol／L乙酸稀释后即可进样，净化回收率为102．8％，法莫曾丁的最低检测浓度为3ng／ml。分析一个样品仅需10min。
RP2 (25 ~ 40μm) was used as the pretreatment column and water was used as the pretreatment mobile phase. With 0.2mol / L acetic acid and 0.2mol / L acetic acid as the cleaning liquid, the plasma was directly injected into the on- line) was used to purify the sample. The mobile phase consisted of Shimpack CLC-ODS (15cm × 6mmID), methanol-acetic acid (0.2mol / L, pH2.7) Standard method for quantitative determination of human plasma concentration of famotidine method. The method to switch the system is simple, the instrument requires a switching valve and two solvent systems. 200μl plasma only. 05mol / L acetic acid can be injected after dilution, purification recovery was 102.8%, the lowest detection rate of famodin 3ng / ml. Analysis of a sample only 10min.