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阔叶植物叶片通常释放少量的挥发物 ,但是当受到害虫取食后植物会释放大量的物质 ,并且其挥发物的指纹图谱与未被损伤的植株相比大不相同 .这些挥发物在植物 -害虫 -害虫天敌之间的关系中起着非常重要的作用 .为了进一步了解昆虫取食植物后挥发物的释放机制 ,作者选用复叶槭 (Acernegundo)和光肩星天牛 (Anoplophoraglabripennis (Motsch .) )为试验材料 ,利用气相色谱和质谱联用 (GC MS)检测挥发物 .在天牛取食后大多数物质在 9h的释放率达到第一个峰值 (β 蒎烯、六氢法尼烯基丙酮、乙酸苯乙酯、苯甲酸甲酯、水杨酸甲酯、syn 2 甲基丁醛肟除外 ) .在经过短时间下降后又逐渐上升 ,至天牛取食后 48h达到最大值 .只有 β 蒎烯、3 蒈烯、桉树脑、D 柠檬烯、trans 香叶基丙酮、苯甲酸甲酯、苯乙酮、苯并噻唑等物质在 72h达到最大 ,而syn 丁醛肟、1 ,3 ,3 三甲基 2 甲烯二氢吲哚在咬食 1h后就达到峰值 .天牛咬食诱导出的复叶槭挥发物 (即在正常植株中未被检测到的物质 )种类随时间的变化逐渐增加 ,在 48h达到 1 7种 .
Broadleaf leaves usually release small amounts of volatiles, but when released by pests, the plant releases a significant amount of material and the fingerprint of its volatiles is quite different from that of unimpaired plants.These volatiles are found in plant- Pest and pest natural enemies.In order to further understand the release mechanism of volatiles after feeding on insects, the authors selected Acernegundo and Anoplophora glabripennis (Motsch.) As test materials , And the volatiles were detected by gas chromatography and mass spectrometry (GC MS). The release rate of most substances reached the first peak at 9h after feeding (β-pinene, hexafarfurkenyl acetone, benzene Methyl benzoate, methyl salicylate, and syn 2 methyl butyraldehyde oxime except), after a short period of time and then gradually increased to 48 h after feeding to maximum, only β-pinene, 3 acenaphthylene, eucalyptol, D limonene, trans geranyl acetone, methyl benzoate, acetophenone, benzothiazole and other substances reached the maximum at 72h, while butyryl oxime, 1,3,3-trimethyl-2 Methane two Hydrogen-indoles peaked at 1 h after biting, and the species of Acer noconsinum (that is, undetectable in normal plants) induced by the bovine seizures gradually increased over time, reaching a peak at 48 h Species.