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目的探讨甲胎蛋白异质体(AFP-L3)在肝细胞癌(hepatocellular carcinoma,HCC)早期诊断中的价值。方法选择186例甲胎蛋白(α-Fetoprotein,AFP)水平为20~400 ng/ml肝病患者的血清,应用微量凝集素亲和吸附离心管分离、洗脱获得AFP-L3,用化学发光免疫分析法检测AFP和AFP-L3含量,计算AFP-L3占血清AFP的百分比即AFP-L3百分数。结果 HCC患者血清中AFP-L3水平及AFP-L3百分数明显高于慢性肝炎(chronic hepatitis,CH)和肝硬化(liver cirrhosis,LC)患者(P<0.01);以AFP-L3百分数≥10%为阳性,HCC患者AFP-L3的阳性率明显高于CH和LC患者。AFP-L3百分数诊断HCC的灵敏度是71.43%,特异度是92.36%。结论检测血清中AFP-L3水平对早期HCC的诊断有重要价值。
Objective To investigate the value of AFP-L3 in the early diagnosis of hepatocellular carcinoma (HCC). Methods A total of 186 serum samples of liver cirrhosis patients with 20-400 ng / ml alpha-Fetoprotein (AFP) were selected and separated by micro-agglutinin affinity chromatography. AFP-L3 was obtained by chemiluminescence immunoassay Method to detect AFP and AFP-L3 content, calculate AFP-L3 percentage of serum AFP, AFP-L3 percentage. Results The serum levels of AFP-L3 and percentage of AFP-L3 in HCC patients were significantly higher than those in patients with chronic hepatitis (CH) and liver cirrhosis (LC) (P <0.01) Positive, HCC patients AFP-L3 positive rate was significantly higher than CH and LC patients. The percentage of AFP-L3 diagnosed HCC was 71.43% and the specificity was 92.36%. Conclusion Detection of serum AFP-L3 levels is of great value in the diagnosis of early-stage HCC.