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目的:研究预防性中药抗流感病毒及其黏膜免疫调节机制。方法:56只雌性SPF级BALB/C小鼠,随机分为造模组(48只)和正常组(8只),建立小鼠流感病毒H1N1/FM/1感染模型,造模成功后随机分为模型组、病毒唑组(0.1 g·kg-1),银翘散组(40 g·kg-1),玉屏风散组(60 g·kg-1),冰香散雾化组(0.6 g·kg-1),冰香散滴鼻组(0.03 g·kg-1),ig给药,5 d后处死小鼠,测定病毒载量,同时进行肺组织病理学检查;检测对气管黏膜树突状细胞(DC)数量(OX-62),成熟度:以CD80,趋化因子激素受体7(CCR7)阳性表达面积表示及抗原识别受体Toll样受体2(TLR2)和TLR4的影响;对鼻通道淋巴组织细胞CD4+,CD8+进行相应的检查。结果:与模型组比较,预防性中药对病毒载量及肺组织病理学有显著的抑制作用,流感病毒感染小鼠气管黏膜OX-62增加,冰香散(雾化)能使流感病毒感染鼠DC数降低,CD80和CCR7明显下降,而病毒唑和银翘散制剂能提升CCR7阳性表达面积,流感病毒感染鼠黏膜TLR2,TLR4表达增强,冰香散能抑制流感病毒感染鼠TLR2的表达,冰香散滴鼻给药能提高CD4+,CD8+的水平,均具有明显的统计学差异(P<0.05,P<0.01)。结论:三类防治流感的中药均有较好的抑制流感病毒作用,其作用可能与其调节呼吸道黏膜免疫作用有关。
Objective: To study the preventive Chinese medicine anti-influenza virus and mucosal immunoregulation mechanism. Methods: Fifty-six female SPF BALB / C mice were randomly divided into model group (n = 48) and normal group (n = 8). The mouse model of influenza H1N1 / FM1 infection was established. The rats in model group were treated with ribavirin (0.1 g · kg-1), Yinqiao San (40 g · kg-1), Yupingfeng San (60 g · kg- g · kg-1) and Bingxian Sanbiao nasal group (0.03 g · kg-1) were given to mice for 5 days. The mice were killed after 5 days. The viral load was measured and lung histopathological examination was performed. The number of dendritic cells (DC) (OX-62), maturation: expressed as the positive expression area of CD80 and chemokine receptor 7 (CCR7) and the expression of the TLR2 and TLR4 Influence; nasal passages lymphoid cells CD4 +, CD8 + corresponding examination. Results: Compared with the model group, the prophylactic traditional Chinese medicine had a significant inhibitory effect on the viral load and lung histopathology. The increase of OX-62 in tracheal mucosa of mice infected with influenza virus and the increase of Bingxiang San (atomization) DC decreased and CD80 and CCR7 decreased significantly, while ribavirin and Yinqiaosan preparations increased the expression of CCR7, the expression of TLR2 and TLR4 increased in mice infected with influenza virus. Bingxian powder could inhibit the expression of TLR2 in mice infected with influenza virus. Hong nasal administration of nasal drops can increase the level of CD4 +, CD8 +, have significant statistical difference (P <0.05, P <0.01). CONCLUSION: The three types of Chinese medicine for preventing and treating influenza all have a better effect of inhibiting influenza virus, which may be related to the regulation of respiratory mucosal immunity.