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目的：观察蝎毒抗癌多肽（ａｎｔｉｎｅｏｐｌａｓｔｉｃｐｏｌｙｐｅｐｔｉｄｅｆｒｏｍＢｕｔｈｕｓｍａｒｔｅｎｓｉｖｅｎｏｍ，ＡＰＢＭＶ）对人低分化鼻咽癌细胞（ＣＮＥ２Ｚ）的毒性作用，从而找出ＡＰＢＭＶ抑制人鼻咽癌的最佳浓度。方法：用ＭＴＴ法测定ＡＰＢＭＶ对人低分化鼻咽癌细胞（ＣＮＥ２Ｚ）的毒性。ＣＮＥ２Ｚ细胞培养于ＲＰＭＩ１６４０培养液中；选增生活跃，形态良好的细胞接种在９６孔培养板。细胞浓度为５×１０４ｍｌ－１。细胞被分为１０个实验组，ＭＭＣ阳性对照组、阴性对照组和８个ＡＰＢＭＶ实验组，实验组的ＡＰＢＭＶ质量浓度分别为１ｍｇ·Ｌ－１，２ｍｇ·Ｌ－１，３ｍｇ·Ｌ－１，４ｍｇ·Ｌ－１，５ｍｇ·Ｌ－１，６ｍｇ·Ｌ－１，７ｍｇ·Ｌ－１，８ｍｇ·Ｌ－１。加药后，细胞培养４８ｈ，然后加入ＭＴＴ１０μｌ·孔－１，继续培养４ｈ，加入２００ｇ／ＬＳＤＳ５００ｇ／ＬＤＭＦ混合溶液溶解细胞内的结晶体，测定５６０ｎｍ和６３０ｎｍ波长处的Ｄ值，并计算生长抑制率（Ｇｒｏｗｔｈｉｎｈｉｂｉｔｉｎｇｒａｔｅ，ＧＩ）。结果：ＡＰＢＭＶ在２～８ｍｇ·Ｌ－１浓度范围内，ＧＩ＜５０％，ＣＴ＞５０％，其中在３～６ｍｇ·Ｌ－１浓度范围内，ＣＴ?
Objective: To observe the toxic effect of antineoplasticpolypeptide fromButhusmartensivenom (APBMV) on human poorly differentiated nasopharyngeal carcinoma cells (CNE2Z), so as to find the optimal concentration of APBMV for inhibiting human nasopharyngeal carcinoma. Methods: The toxicity of APBMV on human poorly differentiated nasopharyngeal carcinoma cells (CNE2Z) was determined by MTT assay. CNE 2Z cells were cultured in RPMI1640 medium; selected active hyperplasia, well-shaped cells were seeded in 96-well plates. The cell concentration was 5 × 104 ml-1. The cells were divided into 10 experimental groups, MMC positive control group, negative control group and 8 APBMV experimental groups. The APBMV concentration in the experimental group was 1 mg · L -1, 2 mg · L -1, 3 mg · L -1, 4 mg · L -1,5 mg · L -1,6 mg · L -1,7 mg · L -1,8 mg · L -1. After dosing, the cells were cultured for 48h, and then added MTT10μl · hole-1, continue to culture 4h, 200g / LSDS 500g / LDMF mixed solution was added to dissolve intracellular crystals, measured at 560nm and 630nm wavelength D value, and calculate the growth inhibition Growth (Growthinhibitingrate, GI). Results: In the range of 2 ~ 8 mg · L-1, APBMV had GI <50% and CT> 50%. In the range of 3 ~ 6 mg · L-1,