论文部分内容阅读
在辣椒(Capsicum annuum)种内进行抗CMV QTL分析。从感病品种Maor与抗病品种Perennial杂交获得180个F3。在美国和以色列的三个试验中用两个病毒株系接种。大部分RFLP和AFLP标记被用作构建遗传图谱,区间分析被用作QTL检测。有4个QTL与抗CMV显著相关。检测到了两个标记对CMV抗性的双基因互作,没有检测到单基因效用。3个试验中检测到控制表现型变异(cmv11.1)的QTL,其变异最大百分比为16%～33%,该QTL与双基因互作有关。这个QTL与L位点连锁,并证实该QTL与抗TMV有关。在Perennial上早期非常有趣的观察到抗CMV感TMV的现象。来自于不相关的种群的一个高世代的回交育种株系3990,被选择用来分析对CMV的抗性,标记覆盖整个基因组,检测到来自Perennial的基因渗入。这些基因渗入的区域中包括4个与抗CMV相关的QTL。在两个基因区域的标记被鉴定与抗CMV的QTL相关,同时也与控制果实重量的QTL相关,另外的育种观察也证实对CMV的抗性来源于Perennial和小果型品种。
Anti-CMV QTL analysis was performed in Capsicum annuum. 180 F3 lines were obtained from the susceptible variety Maor and the resistant variety Perennial. Two strains of virus were inoculated in three trials in the United States and Israel. Most RFLP and AFLP markers were used to construct genetic maps, and interval analysis was used as QTL detection. Four QTLs were significantly associated with anti-CMV. Two markers of CMV resistance were detected for two-gene interactions and no single-gene effect was detected. QTLs controlling phenotypic variation (cmv11.1) were detected in 3 of these experiments, with a maximum percentage variation of 16% -33%. This QTL is associated with a double-gene interaction. This QTL was linked to site L and confirmed that the QTL was associated with anti-TMV. The phenomenon of anti-CMV susceptibility TMV was observed very early on Perennial. A high-generation backcross line 3990 from an unrelated population was selected for analysis of resistance to CMV, with the marker covering the entire genome and the introgression from Perennial detected. Four introgressed QTLs were found in these introgressed regions. The markers in the two gene regions were identified as being associated with the anti-CMV QTLs and also with the QTL controlling the weight of the fruit. Additional breeding observations confirmed that the resistance to CMV was derived from Perennial and the small fruit variety.