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目的:比较雌雄株栝楼根氨基酸含量差异。方法:蛋白经盐酸水解得氨基酸,柱前衍生反相高效液相色谱法检测其含量。结果:雌、雄株栝楼根氨基酸含量为7.35%、7.04%。结论:柱前衍生高效液相色谱法可用于栝楼根氨基酸含量测定。总氨基酸含量雄株略低于雌株;碱性氨基酸含量雄株高于雌株,组氨酸含量约为雌株两倍;必需氨基酸含量无显著差异。
Objective: To compare the difference of amino acid content between the male and female plants. Methods: The amino acids were hydrolyzed by hydrochloric acid and the contents were determined by precolumn derivatization reversed-phase high performance liquid chromatography. Results: The contents of amino acids in male and female plants were 7.35% and 7.04%, respectively. Conclusion: Pre-column derivatization high performance liquid chromatography can be used for determination of amino acids in the root of Rhizoma. The total amino acid content of male plants was slightly lower than that of female plants; the content of basic amino acids was higher than that of female plants, the content of histidine was about twice as that of female plants; the content of essential amino acids had no significant difference.