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采用M13通用接头连接锚定引物,建立了适合于菜薹的荧光微卫星锚定片段长度多态性(MFLP)技术;在此基础上,从360对选择性扩增引物组合中筛选出9对适宜的引物组合,对收集的32份菜薹种质进行等位基因多态性分析;结果显示这些引物组合的扩增产物在32份菜薹种质中的等位基因多态性在10~31之间,平均为17.7个;多态性信息量在0.61~0.98之间,平均0.81。利用获得等位基因多态性进行的遗传相似性分析表明,32份菜薹种质间的相似系数在0.277~0.836之间,平均0.624;基于多态性数据,运用除权配对法(UPGMA)进行聚类分析,将这些菜薹种质材料分为2个组群和4个亚群。这些结果显示荧光MFLP标记技术能有效地发现供试菜薹种质材料的DNA多态性,表明该技术在菜薹遗传特性分析的研究和应用领域具有可行性。
The M13 universal linker was used to ligate the anchor primer to establish a fluorescent microsatellite anchor fragment length polymorphism (MFLP) technique suitable for Cauliflower. On the basis of this, 9 out of 360 pairs of selective amplification primer combinations were screened The results showed that the allele polymorphisms of the amplification products of these primer combinations in 32 accessions of Brassica rapa were between 10 ~ 31, an average of 17.7; polymorphism information between 0.61 to 0.98, an average of 0.81. Genetic similarity analysis based on allelic polymorphism showed that the similarity coefficients of 32 accessions were between 0.277 and 0.836 with an average of 0.624. Based on the polymorphism data, UPGMA Cluster analysis, these cabbage germplasm materials are divided into two groups and four subgroups. These results show that the fluorescence MFLP labeling technology can effectively find the DNA polymorphism of G. bungeana germplasm materials, indicating that this technique is feasible in the research and application of the genetic characteristics of B. incisum.