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目的研究外周血造血干细胞简化冷藏保存方法。方法:用IMDM、白蛋白、血浆三种不同保养液于4℃保存造血干细胞,分别在当天、第3至第6天测其存活率、CD34阳性率及CFU-GM集落形成能力。结果:造血干细胞保存在4℃以不超过72小时为好;保养液以自身血浆及4%白蛋白为佳。
Objective To study the simplified method of cold preservation of peripheral blood hematopoietic stem cells. Methods: Hematopoietic stem cells were stored at 4 ℃ with three different maintenance solutions of IMDM, albumin and plasma. The survival rate, CD34 positive rate and CFU-GM colony formation ability were measured on the 3rd and the 6th day respectively. Results: Hematopoietic stem cells were stored at 4 ° C for no more than 72 hours; maintenance solutions were preferred for their own plasma and 4% albumin.