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中性粒细胞(PMN)是炎性器官损害中的重要介质,从炎症处清除PMN是炎症消散的先决条件,PMN凋亡是非炎性处置PMN的一种机制。细胞凋亡不同于细胞坏死,仅有典型的形态学改变(核固缩以及染色质聚集)和生化标志(DNA裂解),吖啶橙溴化乙啶染色可显示细胞膜完整以及典型的核形态学变化。还可应用DNA胶电泳法作凋亡定量测定。至于PMN的功能性容量可经其生成O_2~-的能量来评估。大戟二萜醇-12-豆蔻酸盐-13乙酸盐(PMA)不经过细胞膜受体而直接激活细胞内
Neutrophils (PMNs) are important mediators of inflammatory organ damage. Removal of PMN from inflammation is a prerequisite for the resolution of inflammation, and PMN apoptosis is a mechanism of noninflammatory treatment of PMNs. Apoptosis is distinct from cell necrosis with only typical morphological changes (nuclear pyknosis and chromatin aggregation) and biochemical markers (DNA cleavage). Acridine orange ethidium bromide staining shows cell membrane integrity as well as typical nuclear morphology Variety. DNA gel electrophoresis can also be used for quantitative determination of apoptosis. As for the functional capacity of PMN, it can be assessed by its energy of producing O 2 -. Incense-12-myristate-13 acetate (PMA) directly activates intracellularly without passing through the cell membrane receptor