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目的研究分离自‘米雅’的酪酸菌(丁酸梭菌)治疗感染性腹泻小鼠的疗效及其安全性,并与链霉素、三黄汤的治疗效果进行比较。方法经大肠杆菌O157:H7感染制备小鼠的腹泻模型,采用灌胃方式进行给药治疗。测定各组小鼠的死亡率,观察小肠和肾脏组织的病理变化。结果①从实验小鼠的死亡率看,酪酸菌组最高;②从实验小鼠小肠组织病理结果看,酪酸菌治疗对降低小肠组织的炎症率有明显疗效;③从肾脏组织的病理结果看,酪酸菌治疗使肾脏组织的炎症率增加,病变程度加重。结论酪酸菌对腹泻小鼠的治疗,对改善小肠上皮组织的病变有明显疗效,但加重了肾脏组织的病变程度,其安全性值得进一步关注。
Objective To study the efficacy and safety of butyric acid bacteria (Clostridium butyricum) isolated from ’Mia’ in the treatment of infectious diarrhea in mice and to compare its efficacy with streptomycin and Sanhuang Decoction. Methods The diarrhea model of mice was prepared by infection with E. coli O157: H7, and the mice were treated by intragastric administration. The mortality of mice in each group was measured, and pathological changes of small intestine and kidney were observed. Results ① From the mortality rate of experimental mice, the highest in the group of butyric acid bacteria; ② from the experimental mouse intestinal histopathological results, the treatment of butyric acid bacteria to reduce inflammation in the intestinal tissue has a significant effect; ③ pathological findings from the kidney, Butyric acid bacteria treatment to increase the rate of inflammation of the kidneys, lesions increased. Conclusion The treatment of diarrhea mice by butyric acid bacteria has obvious curative effect on improving the pathological changes of small intestine epithelium, but it aggravates the degree of renal tissue lesions. Its safety deserves further attention.