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目的探讨CpG岛甲基子表型阳性的散发性大肠癌的临床病理特征。方法采用甲基化特异性PCR方法对71例散发性大肠癌患者行p14、hMLH1、p16、MGMT和MINT1共5个基因启动子甲基化检测,分析CpG岛甲基子表型阳性的散发性大肠癌的临床病理特征。结果 71例散发性大肠癌患者中共检出CpG岛甲基子表型阳性者15例,阳性率为21．1%。CpG岛甲基子表型阳性的散发性大肠癌中右半结肠癌(40．0％比12．5%,P<0．05)、低分化癌(46．7％比14．3％,P<0．05)、淋巴结转移 (86．7％比48．2％,x2=7．112．P<0．05)、Dukes C期或D期(86．7％比50．0％,x2=6．519,P<0．05) 所占比例均显著高于CpG岛甲基子表型阴性者。结论 CpG岛甲基子表型阳性的散发性大肠癌具有右半结肠癌多发、低分化多见、常有淋巴结转移和分期较晚的特点。
Objective To investigate the clinicopathological features of sporadic colorectal cancer with CpG island-like phenotype. Methods The promoter methylation of p14, hMLH1, p16, MGMT and MINT1 gene in 71 patients with sporadic colorectal cancer was detected by methylation-specific PCR. The methylated phenotype of CpG island was analyzed. Colorectal cancer clinicopathological features. Results Fifty-one cases of CpG island-positive phenotype were detected in 71 patients with sporadic colorectal cancer, the positive rate was 21.1%. Right colon cancer (40.0% vs. 12.5%, P <0.05), poorly differentiated cancer (46.7% vs 14.3%, P <0.05) P <0.05), lymph node metastasis (86.7% vs 48.2%, x2 = 7.112.P <0.05), Dukes C or D (86.7% vs. 50.0% x2 = 6.519, P <0.05) were significantly higher than the CpG island methyl subtypes negative. Conclusion CpG island phenotype-positive sporadic colorectal cancer with multiple colon cancer of the right, poorly differentiated more common, often lymph node metastasis and the characteristics of the late stage.