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目的:建立布渣叶总黄酮提取物中表儿茶素、牡荆苷、异牡荆苷、山柰酚-3-O-芸香糖苷、异鼠李素-3-O-葡萄糖苷和水仙苷等6种黄酮类成的HPLC同时测定方法。方法:采用HPLC法,Agilent HC-C_(18)色谱柱(4.6 mm×250 mm,5μm),甲醇-0.05%磷酸水溶液为流动相进行梯度洗脱,检测波长288 nm,柱温35℃,流速0.8 m L·min~(-1)。结果:表儿茶素、牡荆苷、异牡荆苷、山柰酚-3-O-芸香糖苷、异鼠李素-3-O-葡萄糖苷和水仙苷分别在131.200~1 312.000,149.800~1 348.200,131.400~1 051.200,99.175~595.050,109.955~659.730,267.000~1 348.200 ng呈线性关系,平均回收率分别为98.13%,97.41%,96.93%,98.22%,97.37%,97.82%。结论:该方法准确、可靠,重复性好,可用于布渣叶总黄酮提取物的质量评价与控制。
OBJECTIVE: To establish a method for the determination of epicatechin, vitexin, isovitexin, kaempferol-3-O- rutinoside, isorhamnetin-3-O-glucoside, Such as six kinds of flavonoids HPLC simultaneous determination method. Methods: The mobile phase was eluted with a gradient of Agilent HC-C 18 column (4.6 mm × 250 mm, 5 μm) and methanol-0.05% phosphoric acid. The detection wavelength was set at 288 nm and the column temperature was 35 ℃. The flow rate 0.8 m L · min ~ (-1). Results: Epicatechin, vitexin, isovitexin, kaempferol-3-O-rutinoside, isorhamnetin-3-O-glucoside and narcissin were in the range of 131.200 ~ 1 312.000 and 149.800 ~ The average recoveries were 98.13%, 97.41%, 96.93%, 98.22%, 97.37%, 97.82%, respectively. The average recoveries were 98.13%, 97.41%, 96.93%, 97.37%, 97.82%, respectively. Conclusion: The method is accurate, reliable and reproducible. It can be used for the quality evaluation and control of total flavonoids extract from Cloth Leaves.