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为建立一种简便可行的检测ＨＢＶ前Ｃ区１８９６位Ｇ→Ａ突变的方法。采用本室自行构建的ＨＢＶ前Ｃ区１８９６位Ｇ→Ａ突变株和野生株克隆作为标准株，对错配引物ＰＣＲ（ｍｐ－ＰＣＲ）结合限制性片段长度多态性分析（ＲＦＬＰ），作为检测ＨＢＶ前Ｃ区１８９６位Ｇ→Ａ突变株的方法进行了评价。结果显示，该方法操作较为简便，特异性和重复性均较满意，可用于临床和科研
In order to establish a simple and feasible method for the detection of G → A mutation at 1896 of pre-HBV C region. Using the 1896 G → A mutant and the wild-type clone in the precore HBV precore region constructed by ourselves as the standard strain, PCR-RFLP (restriction fragment length polymorphism) analysis (RFLP) The method of 1896 G → A mutant in pre-HBV C region was evaluated. The results show that the method is simple, specific and reproducible are satisfactory, can be used in clinical and research