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目的:探讨参附注射液对糖尿病合并冠心病ZDF模型大鼠的治疗作用及其对PI3K-AKT信号通路的影响。方法:12只SD大鼠为正常对照组,24只Ⅱ型糖尿病合并冠心病的大鼠随机分为模型组和参附注射液组,每组12只。连续给药10d,检测各组大鼠随机血糖、心电图(ECG)J点位移变化、心肌酶学指标、心肌组织超氧化物歧化酶(SOD)和丙二醛(MDA)含量变化,免疫印迹法(Western blot)检测心肌组织中糖酯酰肌醇激酶(PI3K)、磷酸化内皮型一氧化氮合酶(eNOS)及蛋白激酶(Akt)的表达变化。结果:与模型组比较,参附注射液组J点位移、心肌乳酸脱氢酶(LDH)、肌酸激酶(CK)和MDA含量均显著降低(均P<0.05),心肌组织PI3K、磷酸化eNOS和磷酸化Akt蛋白表达量均显著增高(均P<0.05)。结论:参附注射液通过上调激活PI3K-Akt-eNOS信号通路和eNOS蛋白量,保护大鼠心肌损伤病灶,减少心肌细胞的渗出和氧化应激,对糖尿病合并冠心病病症起到一定治疗作用。
Objective: To investigate the therapeutic effect of Shenfu injection on diabetic ZDF model rats and its effect on PI3K-AKT signaling pathway. Methods: Twelve SD rats were normal control group. Twenty-four type Ⅱ diabetic rats with coronary heart disease were randomly divided into model group and Shenfu injection group, with 12 rats in each group. The rats in each group were randomly divided into control group (n = 10) and control group (n = 10). The changes of blood glucose, J point displacement, myocardial enzymological indexes, myocardial SOD and MDA contents were detected by Western blotting (PI3K), phosphorylation of endothelial nitric oxide synthase (eNOS) and protein kinase (Akt) in myocardium were detected by Western blot. Results: Compared with the model group, J-point displacement, myocardial lactate dehydrogenase (LDH), creatine kinase (CK) and MDA content in the Shenfu injection group were significantly decreased (all P <0.05) eNOS and phospho-Akt protein expression were significantly increased (all P <0.05). Conclusion: Shenfu injection can protect myocardium injury of myocardium and reduce cardiomyocyte exudation and oxidative stress by up-regulating PI3K-Akt-eNOS signal pathway and eNOS protein, which plays a role in the treatment of diabetes complicated with coronary heart disease .