论文部分内容阅读
目的:研究芦荟大黄素促进人甲状腺癌细胞系K1凋亡的作用。方法:人甲状腺癌细胞系K1与不同浓度的芦荟大黄素共孵育48h,MTT法检测细胞存活率,并用相差显微镜观察细胞形态学改变。用流式细胞术(检测细胞凋亡相关指标Annexin V/PI)和Hoechst33258染色检测细胞凋亡。结果:芦荟大黄素能够不同程度地抑制人甲状腺癌细胞系K1的生长。芦荟大黄素对K1细胞的IC5(0半数抑制浓度)分别为65mg/ml。Hoechst33258荧光染色和流式细胞术分析K1细胞的结果一致。结论:芦荟大黄素可促进K1细胞凋亡,为芦荟大黄素治疗甲状腺癌提供了依据。
Objective: To study the effect of aloe-emodin on the apoptosis of human thyroid carcinoma cell line K1. Methods: The human thyroid carcinoma cell line K1 was incubated with different concentrations of aloe-emodin for 48h. The cell viability was measured by MTT assay. The cell morphological changes were observed by phase-contrast microscopy. Apoptosis was detected by flow cytometry (Annexin V / PI) and Hoechst33258 staining. Results: Aloe-emodin can inhibit the growth of human thyroid cancer cell line K1 to some extent. Aloe-emodin on K1 cells IC5 (0 half inhibitory concentration) were 65mg / ml. The results of Hoechst 33258 fluorescence staining and flow cytometry analysis of K1 cells were consistent. Conclusion: Aloe-emodin can promote the apoptosis of K1 cells and provide the basis for treatment of thyroid cancer with aloe-emodin.