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目的 筛选与小鼠牙胚发育相关的特异基因。方法 利用随机引物、反转录酶合成特异和非特异探针 ,采用差异显示方法筛选小鼠牙胚cDNA文库 ,挑选阳性克隆并测序。结果 得到 6个阳性克隆 ,经测序证实其中 1个克隆序列与大鼠成釉蛋白序列高度同源 :其中用pTriplEX 3′引物测得的 5 2 6个碱基序列与大鼠成釉蛋白基因 5′端的 32 5 80序列有 4 97个碱基一致 ,5′引物测得的 5 6 7个碱基序列与大鼠成釉蛋白基因 3′端的 12 85 185 4序列有 5 33个碱基一致。结论 筛选到小鼠釉基质特异蛋白———成釉蛋白基因的cDNA全序列。
Objective To screen specific genes related to mouse tooth germ development. Methods Using random primers and reverse transcriptase, specific and non-specific probes were synthesized. The mouse tooth germ cDNA library was screened by differential display, and the positive clones were selected and sequenced. Results Six positive clones were obtained. One of the cloned sequences was confirmed to be highly homologous to the rat ameloblastin sequence by sequencing. Among them, the 526 base sequences of pTriplEX 3 ’primer and the rat amelogenin gene 5 There are 4 97 bases in the 32 5 80 sequence of the end and 5 33 bases in the 5 6 7 base sequence measured by the 5 ’primer and 12 85 185 4 sequences of the 3’ end of the rat ametryin gene. Conclusion The full-length cDNA of mouse glaucoma-specific protein, amelogenin gene, was screened out.