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对海洋石油降解菌群DC10的降解特性进行了研究,研制了基于该菌群的降解菌剂,并用于大连实际溢油岸滩生物修复实验,考察了降解菌剂对潮间带和潮上带油污的降解作用,通过分析C17/藿烷、C18/藿烷及总烷烃和总芳烃的降解率来评价其降解效果。降解菌群DC10在实验室条件下对石油的降解率高于各组成菌株,一周时间的石油降解率比对照提高了60%左右,能降解大部分的烷烃和芳烃。以DC10冻干菌粉辅以营养盐溶液研制降解菌剂,该菌剂在大连岸滩油污生物修复试验中显示出显著的降解效果。在为期12d的潮间带油污生物修复试验中,喷洒菌剂处理的C17/藿烷和C18/藿烷降解率相对于自然风化处理分别提高了40%和30%,而总烷烃和总芳烃降解率分别提高了80%和72%。在为期85d的潮上带油污生物修复试验中,从C17/藿烷和C18/藿烷的降解率来看,喷洒菌剂处理对油污的降解程度仅略高于自然风化,但总烷烃和总芳烃的降解率分别提高了近30%和20%。
The biodegradation characteristics of offshore oil degradation strain DC10 were studied, and the biodegradable bacteria agent based on this strain was developed and applied to the actual beach oil remediation experiments in Dalian. The effects of degrading agents on the intertidal and tidal range The degradation of oil pollution was evaluated by analyzing the degradation rate of C17 / hopane, C18 / hopane and total alkanes and total aromatics. Degradation flora DC10 degradation rate of petroleum under laboratory conditions higher than the composition of the strain, a week of petroleum degradation rate than the control increased by about 60%, can degrade most of the alkanes and aromatics. DC10 freeze-dried bacterial powder supplemented with nutrient solution was used to develop the bacteriostat. The fungicide showed significant degradation effect in the bioremediation of Dalian shore beach oil. In the intertidal greywater bioremediation test for 12 days, the degradation rates of C17 / hopane and C18 / hopane treated by spraying fungicides were increased by 40% and 30% respectively compared with the natural weathering treatment, while the total alkanes and total aromatics were degraded Rate increased by 80% and 72% respectively. In the 85d wet-tainted bioremediation experiment, from the degradation rate of C17 / hopane and C18 / hopane, the extent of degradation of oil pollution by spray-on agent was only slightly higher than that of natural weathering, but the total alkanes and total Aromatics degradation rate increased by nearly 30% and 20%.