论文部分内容阅读
目的:探讨转录因子FoxM1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)中的表达及其临床意义。方法:应用组织芯片技术和免疫组织化学方法检测FoxM1蛋白在83例ccRCC和30例癌旁肾组织中的表达;应用逆转录聚合酶链反应法(RT-PCR)检测FoxM1mRNA在30例ccRCC和对应的癌旁肾组织中的表达,分析其与ccRCC患者临床病理特征及预后的关系。结果:FoxMl蛋白在ccRCC组织和癌旁肾组织中的阳性表达率分别为79.5%(66/83)和10.0%(3/30),差异有统计学意义(P<0.001)。FoxM1蛋白表达与ccRCC患者的年龄、性别无关(P>0.05),而与原发肿瘤T分期(P<0.001)、组织学分级(P<0.001)、淋巴结转移(P<0.001)、远处转移(P=0.001)和TNM分期(P<0.001)相关。RT-PCR结果显示FoxM1mRNA在ccRCC和癌旁组织中的相对表达量分别为0.652±0.026和0.327±0.043,差异有统计学意义(P<0.05)。FoxM1mRNA表达与ccRCC患者原发肿瘤T分期(P<0.001)、组织学分级(P=0.004)、淋巴结转移(P=0.006)、远处转移(P=0.005)和TNM分期(P<0.001)有关。Kaplan-Meier生存分析显示,FoxM1蛋白高表达组患者的总生存时间短于低表达组患者,差异有显著统计学意义(Log-rank=46.117,P<0.001)。Cox多因素风险比例模型显示,原发肿瘤T分期(P=0.009)、远处转移(P=0.006)和FoxM1表达情况(P=0.001)是影响ccRCC患者预后的独立因素。结论:FoxM1在ccRCC组织中高表达,可能与ccRCC的进展有关;其表达状态是ccRCC患者独立预后因素。
Objective: To investigate the expression of FoxM1 in clear cell renal cell carcinoma (ccRCC) and its clinical significance. Methods: The expression of FoxM1 protein in 83 cases of ccRCC and 30 cases of para-carcinoma tissues was detected by tissue microarray and immunohistochemistry. The mRNA and protein expressions of FoxM1 mRNA in 30 cases of ccRCC and corresponding tissues were detected by reverse transcription-polymerase chain reaction (RT-PCR) Of paracancer renal tissue expression, analysis of its clinical and pathological features and prognosis of ccRCC patients. Results: The positive rates of FoxM1 protein in the tissues of both ccRCC and paracancer tissues were 79.5% (66/83) and 10.0% (3/30), respectively. The difference was statistically significant (P <0.001). The expression of FoxM1 was not associated with the age and gender of patients with ccRCC (P> 0.05), but significantly correlated with T stage (P <0.001), histological grade (P <0.001), lymph node metastasis (P = 0.001) and TNM stage (P <0.001). The results of RT-PCR showed that the relative expression levels of FoxM1 mRNA in ccRCC and adjacent tissues were 0.652 ± 0.026 and 0.327 ± 0.043, respectively, with statistical significance (P <0.05). FoxM1 mRNA expression was correlated with T stage (P <0.001), histological grade (P = 0.004), lymph node metastasis (P = 0.006), distant metastasis (P = 0.005) and TNM stage . Kaplan-Meier survival analysis showed that the overall survival time of patients with high FoxM1 protein expression was shorter than that of patients with low expression, the difference was statistically significant (Log-rank = 46.117, P <0.001). Cox multivariate risk proportional model showed that T stage (P = 0.009), distant metastasis (P = 0.006) and FoxM1 expression (P = 0.001) were independent prognostic factors in patients with primary ccRCC. Conclusion: FoxM1 is overexpressed in ccRCC tissues, which may be related to the progress of ccRCC. The expression of FoxM1 is an independent prognostic factor in patients with ccRCC.