番茄晚疫病菌是一种寄生性较强的病原真菌 ,分离纯化难度较大。采用选择性培养基 (黑麦粉 5 0g ,琼脂1 5 g ,水 1 0 0 0ml,灭菌后加入利福平 2 0mg ,氨卞青霉素 2 0 0mg和 70 %五氯硝基苯 1 0 0mg) ,选择刚发病的症状典型的病叶 ,在显微镜下挑取晚疫病菌菌丝顶端 ,移植到平板上培养 ,待菌丝呈放射性生长 2～ 3d后 ,再将菌落边缘单菌丝的尖端连同培养基切下 ,置于斜面黑麦基础培养基上 ,可获得番茄晚疫病菌纯菌种。 Tomato Phytophthora infestans is a parasitic strong pathogenic fungi, separation and purification more difficult. Selective medium (rye flour 50 g, agar 15 g, water 10 000 ml, rifampicin 200 mg, ampicillin 200 mg and 70% pentachloronitrobenzene 100 mg) , Select the symptoms of the disease just typical disease leaves, picked the top of the mycelia of Phytophthora infestans under the microscope, transplanted to the plate culture until the mycelium was radioactive growth of 2 ~ 3d, and then the tip of the single colony edge together with The medium was cut and placed on a slant rye basal medium to obtain a pure tomato species of Phytophthora infestans.