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十字花科黑腐病菌(Xanthomonas campestris pv.campestris,Xcc)是引起十字花科植物黑腐病的病原细菌,其通过不同的分泌系统将效应物蛋白或细胞毒素转运进真核宿主细胞产生病害,对农业生产造成巨大的经济损失。本研究利用GUS融合报告系统,考察了常见的几种不同金属离子(Zn2+,Mn2+,Ca2+,Fe2+)对Xcc 8004不同致病系统装置基因表达的影响。结果表明,在1滋mol/L~1 mmol/L浓度区间,Zn2+和Mn2+同时对Xcc域型分泌系统(T2SS)和芋型分泌系统(T3SS)有抑制作用;在大于0.01 mmol/L浓度时,Ca2+特异性抑制T3SS;Fe2+同时抑制T2SS、T3SS和郁型分泌系统(T4SS)装置基因的表达。金属离子Zn2+、Mn2+、Ca2+和Fe2+在不同浓度下对Xcc的致病系统均有抑制作用。本研究为分析病原细菌不同分泌系统响应金属离子的模式奠定了基础。
Xanthomonas campestris pv. Campestris (Xcc) is a pathogenic bacterium that causes black rot in cruciferous plants, which transports effector proteins or cytotoxins into eukaryotic host cells through different secretion systems to cause disease, Causing huge economic losses to agricultural production. In this study, we used the GUS fusion reporter system to investigate the effects of several common metal ions (Zn2 +, Mn2 +, Ca2 +, Fe2 +) on the gene expression of different pathogenic system devices in Xcc 8004. The results showed that both Zn2 + and Mn2 + could inhibit T2SS and T3SS in the concentration range of 1 mmol / L ~ 1 mmol / L. When the concentration was more than 0.01 mmol / L, , Ca2 + specific inhibition of T3SS; Fe2 + inhibition of T2SS, T3SS and depression type secretion system (T4SS) device gene expression. The metal ions Zn2 +, Mn2 +, Ca2 + and Fe2 + all inhibited the pathogenic system of Xcc under different concentrations. This study laid the foundation for the analysis of different secretion systems of pathogenic bacteria in response to metal ions.