Angelicae Pubescentis Radix (APR),a widely used traditional Chinese medicine (TCM),is mainly used to treat rheumatism and headache diseases.Angelol B is one of the bioactive constituents of APR with significant anti-inflammatory activity.This paper is aimed to illustrate the metabolites of angelol B in vivo.To achieve this objective,a metabolomics approach based on a rapid and accurate UPLC-Q-TOF-MS method was used to detect the metabolites of Angelol B in rat.A gradient elution system (ACN and 0.1％ formic acid water) equipped with an Agilent SB-C18 column (1.8 μm,2.1 mm × 50 mm) to complete the separation.Scanning area at m/z 100-800 operated on an electrospray ionization (ESI).The data were collected in both positive and negative ion mode and analyzed by the Masslynx 4.1 and SIMCA 13.0 software.A total of 31 metabolites including 20 phase Ⅰ and 11 phase Ⅱ[metabolites were identified.Their structure and fragmentation process were deduced based on the MS and MS/MS data.All of thirty-one metabolites are new compounds based on the search of SCI-Finder database.