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卒中后的康复治疗能改善神经环路的重塑,促进功能恢复。但人们对其潜在的细胞分子机制却知之甚少。特别是康复训练对在大脑局部缺血的病理生理过程中扮演着重要角色的胶质细胞的影响,一直不甚明了。现在,作者设计一项实验来检测康复训练对光化学诱导的局灶性脑缺血损伤灶周围(梗死灶位于大鼠前肢感觉运动皮质功能区)的不同胶质细胞种群增殖和存活的影响。造模成功后,标准组小鼠即开始在标准笼中接受每日定期的针对损伤前肢的功能训练,强化组大鼠转移到强化环境中饲养,抓取组大鼠仍然放在标准笼中未给予进一步的治疗。作者通过感觉运动行走测试来评价功能恢复情况,并于梗死后2-6d给予BrdU以标记检测增殖的细胞。分贝在梗死后的第10天和第42天,用免疫细胞化学方法对病灶周围增殖和存活的星形胶质细胞、小胶质细胞/巨噬细胞和成熟或未成熟的少突胶质细胞进行定量检测。结果作者发现,强化环境和抓取训练都能显著提高损伤前肢的功能恢复程度。此外,这些治疗能显著减少梗死灶周围小胶质细胞/巨噬细胞的增殖,其中对损伤前肢的每日训练还能明显提高新生星形胶质细胞的存活率。因此,作者的数据证明,皮质梗死后的康复治疗不仅能促进功能恢复,而且显著影响着病灶周围区胶质细胞的反应。
Post-stroke rehabilitation can improve the remodeling of the neural circuit and promote functional recovery. However, little is known about the underlying cellular molecular mechanisms. In particular, the impact of rehabilitation training on glial cells, which play an important role in the pathophysiology of cerebral ischemia, remains unclear. Now, we designed an experiment to examine the effects of rehabilitation training on the proliferation and survival of different glia cells surrounding photochemically-induced focal cerebral ischemic lesions (infarct sites located in the functional motor cortex of the forelimb of the rat). After successful modeling, the standard group of mice began to receive regular daily routine training on injured forelimbs in standard cages. The rats in intensive group were transferred to intensive enviornment, and the rats in grazing group were still in standard cages Give further treatment. The authors evaluated functional recovery by sensory walking test and BrdU was given 2-6 days after infarction to label proliferating cells. Decibels On day 10 and day 42 after infarction, astrocytes, microglia / macrophages and mature or immature oligodendrocytes that proliferate and survive around the lesion are immunocytochemically treated Quantitative detection. Results The authors found that both enhanced environmental and crawling training significantly increased the functional recovery of injured forelimbs. In addition, these treatments significantly reduced the proliferation of microglial / macrophages surrounding the infarct, with daily training of injured forelimbs significantly improving the viability of nascent astrocytes. Therefore, the authors’ data demonstrate that rehabilitation after cortical infarction not only promotes functional recovery but also significantly affects the response of glial cells around the lesion.