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目的探讨原发前列腺癌模型的早期快速建模方法,并研究其基本特征。方法预实验后,选用BALB/C雄性小鼠30只,随机分为模型组和对照组,每组15只。模型组去势后经下腹手术行前列腺内原位注射大剂量MNU,100mg/kg,每2周1次,连续4次。同时经腹腔注射睾酮,12.5mg/kg·d-1。对照组作假手术和注射等量生理盐水。实验结束后解剖观察前列腺等盆腔器官形态并作病理检查,鼠血清作SELDI-TOF-MS、睾酮和VEGF等检测,数据作SPSS13.0统计学分析。结果模型组早期癌变发生率为92.86%,表现为腺体各级不典型增生和上皮内瘤变,腺体紊乱,细胞异型性明显。小鼠血清VEGF高于对照组(P>0.05),睾酮明显升高(P<0.05),SELDI筛选出25个异常蛋白。结论去势小鼠重复多次前列腺内原位注射大剂量MNU并辅助睾酮,可在较短的2个月内建立原发早期癌变模型。在建模方法和模型研究上,值得进一步探讨。
Objective To investigate the early rapid modeling method of primary prostate cancer and to study its basic characteristics. Methods After pre-experiment, 30 male BALB / C mice were randomly divided into model group and control group, with 15 rats in each group. In the model group, high-dose MNU was injected into the prostatic gland via the lower abdomen after the castration. The rats were anesthetized with 100mg / kg MNU once a week for 4 times. At the same time, intraperitoneal injection of testosterone, 12.5mg / kg · d-1. The control group was sham operated and injected with the same amount of saline. At the end of the experiment, the morphology of pelvic organs such as prostate was dissected and pathological examination was performed. The serum was analyzed by SELDI-TOF-MS, testosterone and VEGF. The data were analyzed by SPSS 13.0. Results The incidence of early stage carcinogenesis in model group was 92.86%, which showed atypical hyperplasia and intraepithelial neoplasia at all levels of gland, glandular disorder and cell atypia. Serum VEGF of mice was higher than that of the control group (P> 0.05), testosterone was significantly increased (P <0.05), and 25 abnormal proteins were screened by SELDI. Conclusion Ovariectomized mice were injected with large doses of MNU and assisted testosterone in situ to establish a primary model of early canceration in a short period of 2 months. In the modeling method and model research, it is worth to further explore.