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目的:探讨黄芪(Astragalus)对新生儿窒息后血清诱导人肾小管上皮细胞(HK-2)TLR4/MyD88(Toll-like receptors 4/myeloid differentiation factor 88)表达的影响。方法:以人HK-2为研究对象,分为对照组、窒息组、黄芪组,以200 ml/L窒息血清作为攻击因素,分别检测如下指标:倒置相差显微镜观察细胞形态变化;流式细胞仪(Flow Cytometry,FCM)检测各组细胞TLR4特异性荧光抗体阳性结合率表达情况;采用免疫组织化学法(SP法)检测各组细胞MyD88表达情况。结果:与对照组相比较,窒息组细胞形态变化显著,TLR4特异性荧光抗体阳性结合率和MyD88明显增加;与窒息组相比较,黄芪组细胞形态明显改善,TLR4特异性荧光抗体阳性结合率和MyD88的表达明显减少,差异具有统计学意义(P<0.05)。结论:黄芪具有减轻新生儿窒息后血清诱导的人肾小管上皮细胞TLR/MyD88表达的作用。
Objective: To investigate the effect of Astragalus on the expression of Toll-like receptors 4/myeloid differentiation factor 88 in human renal tubular epithelial cells (HK-2) after neonatal asphyxia. METHODS: Human HK-2 was divided into control group, asphyxia group and astragalus group. 200 ml/L asphyxiation serum was used as the attacking factor. The following indexes were detected: inverted phase contrast microscope to observe the changes of cell morphology; flow cytometry Flow cytometry (FCM) was used to detect the expression of TLR4-specific fluorescent antibody positive cells in each group. Immunohistochemistry (SP method) was used to detect the expression of MyD88 in each group. RESULTS: Compared with the control group, asphyxiation group showed significant changes in cell morphology, TLR4-specific fluorescent antibody positive binding rate and MyD88 increased significantly; compared with the asphyxiation group, the cell morphology of the Astragalus group was significantly improved, and the TLR4-specific fluorescent antibody-positive binding rate was comparable to that of the control group. The expression of MyD88 was significantly decreased and the difference was statistically significant (P<0.05). Conclusion: Astragalus has the effect of reducing serum-induced TLR/MyD88 expression in human renal tubular epithelial cells after neonatal asphyxia.