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目的 建立一种获得高产量子宫内膜腺上皮细胞和基质细胞的分离培养方法。方法 通过胶原酶消化法、系列过滤法及贴壁纯化等技术分离纯化培养人子宫内膜腺上皮细胞和基质细胞 ,并通过光镜及免疫细胞化学染色进行鉴定。结果 腺上皮细胞呈蝌蚪形 ,漩涡状生长。角蛋白免疫细胞化学染色阳性 ,纯度约 90 %。基质细胞呈梭形或多角形 ,平行状生长。波形蛋白染色反应阳性 ,纯度达 95 %。每例子宫肌瘤切除标本可获得 (10～2 5 )× 10 6 原代基质细胞和 (4～ 6 )× 10 6 原代腺上皮细胞 ,每例诊断性刮宫获得的原代细胞数约为前者的 1/ 2～2 / 3。结论 改良培养程序可获得高产量的纯化的子宫内膜腺上皮细胞和基质细胞。
Objective To establish a method for isolation and culture of high-yield endometrial glandular epithelial cells and stromal cells. Methods Human endometrial glandular epithelial cells and stromal cells were isolated and purified by collagenase digestion, serial filtration and adherent purification, and were identified by light microscopy and immunocytochemical staining. Results glandular epithelial cells were tadpole-shaped, swirling growth. Keratin immunocytochemistry positive, purity of about 90%. Stromal cells spindle or polygon, parallel growth. Vimentin positive staining, purity of 95%. Each myomectomy specimen obtained (10 ~ 2 5) × 10 6 primary stromal cells and (4 ~ 6) × 10 6 primary glandular epithelial cells, the number of primary cells obtained in each case of diagnostic curettage is about The former 1/2 ~ 2/3. Conclusion The improved culture program can obtain high yields of purified endometrial glandular epithelial cells and stromal cells.