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建立了蜂蜜中果糖、葡萄糖、蔗糖和麦芽糖的超高效合相色谱-质谱(UPC2-MS)快速检测的方法。样品先用少量的水溶解,再用正己烷/异丙醇(1:1,V/V)溶解,经ACQUITY UPC2BEH(100 mm×3.0 mm,1.7μm)色谱柱分离,以超临界CO2-甲醇(含0.1%NH3·H2O,V/V)为流动相进行梯度洗脱,流速为1.8 m L/min,通过质谱检测器在负离子电喷雾模式下对目标化合物进行分析,外标法定量。检测结果表明:果糖、葡萄糖、蔗糖和麦芽糖在2~150 mg/L范围线性关系良好,R2>0.9990,定量限(LOQ,S/N≥10)为0.22~0.42 mg/L,高、中、低3个浓度水平的加标平均回收率在90.0%~107.5%范围,相对标准偏差(RSD,n=6)均小于4.0%。该方法简单快速,分离效果好,为蜂蜜中果糖、葡萄糖、蔗糖和麦芽糖的分离测定提供了新的色谱技术平台。
A rapid method for the rapid determination of fructose, glucose, sucrose and maltose in honey by ultra performance-coupled gas chromatography-mass spectrometry (UPC2-MS) was established. The sample was dissolved in a small amount of water and then dissolved in n-hexane / isopropanol (1: 1, V / V). The sample was separated on a ACQUITY UPC2BEH (100 mm × 3.0 mm, 1.7 μm) column using supercritical CO2-methanol (0.1% NH3 · H2O, V / V) as mobile phase at a flow rate of 1.8 m L / min. The target compounds were analyzed by mass spectrometry in negative ion electrospray mode. The results showed that the linear relationship between fructose, glucose, sucrose and maltose in the range of 2 ~ 150 mg / L was good, R2> 0.9990, LOQ, S / N≥10 was 0.22 ~ 0.42 mg / The spiked average recoveries of the three low concentration levels ranged from 90.0% to 107.5%, and the relative standard deviations (RSD, n = 6) were all less than 4.0%. The method is simple and rapid with good separation effect and provides a new chromatographic platform for the separation and determination of fructose, glucose, sucrose and maltose in honey.