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目的 探究姜黄素联合顺铂对肺癌A549细胞侵袭转移的影响并探讨其对基质金属蛋白酶-9(matrix metalloproteinase 9,MMP-9)、上皮钙黏素(E-cadherin)等与侵袭转移相关蛋白的影响。方法 运用四甲基偶氮唑盐法检测姜黄素及顺铂两种药单用及联用后对肺癌细胞增殖的影响;运用Transwell小室测定姜黄素、顺铂及两药联用对肺癌细胞侵袭转移的影响;用Western blot检测姜黄素及顺铂单用及联用后肺癌细胞内MMP-9、E-cadherin蛋白的表达。结果 5、10、20、40μmol/L的姜黄素对肺癌A549细胞的增殖抑制率分别为6.50%±1.06%、11.70%±0.88%、22.97%±0.82%、27.93%±0.94%;四种浓度不相同的姜黄素组与对照组相比,差异有统计学意义(P<0.01);四种浓度不相同的姜黄素组之间相比,差异有统计学意义(P<0.05)。1、2、4mg/L的顺铂对肺癌A549细胞的增殖抑制率分别为7.12%±0.86%、20.07%±1.14%、26.88%±0.51%;三种浓度不相同的顺铂组与对照组相比,差异有统计学意义(P<0.01),三种浓度不相同顺铂组之间相比,差异有统计学意义(P<0.01)。姜黄素(2Oμmol/L)联用顺铂(1 mg/L)、姜黄素(20μmol/L)联用顺铂(2 mg/L)、姜黄素(20μmol/L)联用顺铂(4 mg/L)对肺癌A549细胞的增殖抑制率分别是28.37%±0.57%、39.72%±0.64%、46.27%±0.86%;联合用药组和对照组相比,差异有统计学意义(P<0.01);联合用药组与单药组比较,差异有统计学意义(P<0.01)。姜黄素组(20μmol/L)、顺铂组(2 mg/L)、联合组(姜黄素20μmol/L+顺铂2 mg/L)对肺癌A549细胞的侵袭抑制率分别38.62%±0.23%、36.52%±0.33%、63.78%±0.59%;用药组与对照组比较,差异有统计学意义(P均<0.01);联合组与单一用药组比较,差异有统计学意义(P均<0.01)。对照组、姜黄素组(20μmol/L)、顺铂组(2 mg/L)、联合组(姜黄素20μmol/L+顺铂2 mg/L)肺癌细胞内蛋白的灰度值比值分别是:MMP-9,0.768±0.047、0.654±0.104、0.684±0.008、0.444±0.104;E-cadherin,0.603±0.170、0.792±0.050、0.784±0.045、0.879±0.110。药物组与对照组比较,差异有统计学意义(P均<0.01),联合组与单药组比较,差异有统计学意义(P均<0.05)。结论 姜黄素及顺铂联用可以抑制肺癌A549细胞的侵袭与转移,其机制可能与下调MMP-9蛋白,增加E-cadherin蛋白相关。
Objective To investigate the effect of curcumin and cisplatin on the invasion and metastasis of lung cancer A549 cells and to investigate its effect on the expression of matrix metalloproteinase 9 (MMP-9), E-cadherin, influences. Methods The effects of curcumin and cisplatin alone or in combination on proliferation of lung cancer cells were detected by MTT method. The effects of curcumin, cisplatin and two drugs on invasion of lung cancer cells Western blot was used to detect the expression of MMP-9 and E-cadherin protein in curcumin and cisplatin alone and in combination. Results The inhibitory rates of 5, 10, 20 and 40 μmol / L curcumin on A549 cells were 6.50% ± 1.06%, 11.70% ± 0.88%, 22.97% ± 0.82% and 27.93% ± 0.94% The differences were statistically significant (P <0.01) between different curcumin groups and the control group (P <0.01). There was significant difference between the four curcuminoids groups (P <0.05). The inhibitory rates of 1,2,4 mg / L cisplatin on the proliferation of lung cancer A549 cells were 7.12% ± 0.86%, 20.07% ± 1.14% and 26.88% ± 0.51%, respectively. Three different concentrations of cisplatin and control group (P <0.01). There was a significant difference between the three concentrations of cisplatin group (P <0.01). Curcumin (20 mg / L), cisplatin (2 mg / L) and curcumin (20 μmol / L) combined with cisplatin / L) on the proliferation of lung cancer A549 cells were 28.37% ± 0.57%, 39.72% ± 0.64% and 46.27% ± 0.86%, respectively. There was significant difference between the combination group and the control group (P <0.01) ; The combination group and single drug group, the difference was statistically significant (P <0.01). The inhibition rates of curcumin (20μmol / L), cisplatin (2mg / L) and combination group (curcumin 20μmol / L and cisplatin 2mg / L) on lung cancer A549 cells were 38.62% ± 0.23% and 36.52 % ± 0.33% and 63.78% ± 0.59% respectively. There was significant difference between the treatment group and the control group (all P <0.01). The difference between the combination group and the single treatment group was statistically significant (P <0.01). The gray value ratio of control group, curcumin group (20μmol / L), cisplatin group (2mg / L) and combination group (curcumin 20μmol / L + cisplatin 2 mg / L) -9,0.768 ± 0.047,0.654 ± 0.104,0.684 ± 0.008,0.444 ± 0.104; E-cadherin, 0.603 ± 0.170,0.792 ± 0.050,0.784 ± 0.045,0.879 ± 0.110. There was significant difference between the drug group and the control group (P <0.01). The difference between the combination group and the single drug group was statistically significant (P <0.05). Conclusion The combination of curcumin and cisplatin can inhibit the invasion and metastasis of lung cancer A549 cells. The mechanism may be related to down-regulating MMP-9 protein and increasing E-cadherin protein.