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构建一种基于离子液体/纳米材料及亚甲基蓝复合膜的新型电化学免疫传感界面。该界面以碳纳米管/壳聚糖/离子液体复合物为基质,随后利用静电作用将亚甲基蓝与纳米金交替吸附于基质上并利用外层纳米金固定抗体分子。采用电化学阻抗及循环伏安法考察了界面的逐层修饰过程。以鼠IgG为免疫物质模型,在优化的实验条件下,亚甲基蓝的峰电流改变与鼠IgG浓度在5~50ng/mL和50~250ng/mL范围内存在良好的线性关系。
A Novel Electrochemical Immunosensing Interface Based on Ionic Liquids / Nanomaterials and Methylene Blue Composite Films. The interface takes the carbon nanotube / chitosan / ionic liquid composite as a matrix, and then uses the electrostatic interaction to alternately adsorb methylene blue and nano gold on the substrate and fix the antibody molecules with the outer nano gold. Electrochemical impedance and cyclic voltammetry were used to investigate the layer-by-layer modification of the interface. Under the optimal experimental conditions, the change of peak current of methylene blue showed a good linear relationship with the rat IgG concentration in the range of 5 ~ 50ng / mL and 50 ~ 250ng / mL.