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核黄素(riboflavin)即维生素B2,是氢传递体黄素蛋白的辅基.人体所需的核黄素主要来源于膳食,缺乏则可致口舌炎、眼球多血管症等疾病.在水溶液中,核黄素在光照下可吸收光子生成黄素半醌,黄素半醌可将多余的电子转移给水中的氧(O_2),使其形成活性氧.活性氧是一类具有较高反应活性的自由基.近年已证实活性氧有诱导多种癌细胞凋亡的作用;最近有报道显示核黄素在光照下可诱导体外培养的眼球晶体变混浊.我们的研究工作发现核黄素+光照可显著抑制人胃腺癌细胞MGC 80-3生长并诱导细胞凋亡,本文以该细胞株为对象,研究核黄素+光照诱导胃癌细胞凋亡的效应及可能机制,以探讨核黄素光化学反应在光动力学治疗肿瘤上的应用前景.方法:台盼蓝拒染计数法、Giemsa染色法和 Hoechst 33258染色法分别观察核黄素+光照对 MGC80-
Ribavirin (riboflavin) is vitamin B2, which is the auxiliary group of the hydrogen transporter flavoprotein. The riboflavin required by the body is mainly derived from the diet. In the absence of it, it can cause oral glossitis, ocular polyangiopathy and other diseases. In aqueous solution Riboflavin can absorb photon to generate flavin semi-antimony under illumination, and the flavin semi-stilbene can transfer excess electrons to oxygen (O 2 ) in water to form active oxygen. Reactive oxygen species are highly reactive. In recent years, it has been confirmed that reactive oxygen species can induce the apoptosis of various cancer cells; recently, it has been reported that riboflavin can induce opacification of cultured ocular lens in vitro under light irradiation. Our research found that riboflavin + light It can significantly inhibit the growth of human gastric adenocarcinoma cell MGC 80-3 and induce apoptosis. In this study, we investigated the effect and possible mechanism of riboflavin + light-induced apoptosis in gastric cancer cells to investigate the photochemical reaction of riboflavin. Application prospects in photodynamic therapy of tumors. Methods: Trypan blue exclusion dye counting method, Giemsa staining method and Hoechst 33258 staining method were used to observe riboflavin + light on MGC80-