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目的探讨姜黄素对多巴胺能细胞的保护作用及机制。方法采用神经毒素鱼藤酮(1μmol/L)处理经神经生长因子(NGF)(50 ng/ml×7)诱导分化过的PC12细胞,并在处理前6 h加入1μmol/L的姜黄素进行干预,MTT法检测细胞活力,荧光分光光度法检测细胞内活性氧水平,比色法检测还原型谷胱甘肽(GSH)含量。结果 NGF诱导后的PC12细胞经1μmol/L鱼藤酮处理24 h后,细胞活力下降至对照组的57.1%,细胞内活性氧水平为对照组的189%,而GSH水平显著下降(P<0.05);姜黄素干预后,与鱼藤酮组相比,细胞活力和GSH含量显著提高,活性氧水平显著降低(P<0.05)。结论姜黄素对多巴胺细胞具有保护作用,其机制与抗氧化活性有关。
Objective To investigate the protective effect of curcumin on dopaminergic cells and its mechanism. Methods PC12 cells differentiated by nerve growth factor (NGF) (50 ng/ml×7) were treated with neurotoxin rotenone (1 μmol/L), and 1 μmol/L curcumin was added 6 hours before treatment to intervene. MTT The viability of the cells was detected by the method. The level of reactive oxygen species in the cells was detected by fluorescence spectrophotometry. The content of reduced glutathione (GSH) was measured by colorimetric method. Results After treated with 1μmol/L rotenone for 24 h, the cell viability of NGF-induced PC12 cells decreased to 57.1% of the control group. The level of reactive oxygen in the cells was 189% of the control group, and the GSH level was significantly decreased (P<0.05). After curcumin intervention, compared with rotenone group, cell viability and GSH content were significantly increased, and active oxygen level was significantly decreased (P<0.05). Conclusion Curcumin has a protective effect on dopamine cells, and its mechanism is related to its antioxidant activity.