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为了了解肉鸡的健康状况以便对其进行食品安全的风险评估提供依据,本研究从北京某肉鸡屠宰场采集了300份肝、200份脾和100份腺胃,采用HE染色方法对各组织进行病理学观察,用免疫组织化学方法对肝组织中的禽白血病病毒J亚群(ALV-J)gp85特异性抗原进行检测,采用PCR技术对肝组织中的ALV RNA进行检测。结果显示,组织病理学观察可见肝中髓样细胞团块检出率为43%,淋巴细胞浸润病变占75%;脾见有少量淋巴细胞发生坏死、排空;52%腺胃黏膜坏死脱落,25%腺胃黏膜下有淋巴细胞浸润。免疫组织化学观察到肝样品中的ALV-J gp85抗原阳性率为61.7%,脾中阳性率为66.5%,腺胃样品阳性率为52.6%。PCR检测到ALV-J gp85特异性片段PCR产物阳性率为30.5%。利用Gen Bank比对发现与各株ALV-J的同源性为85%~90%。研究结果表明,该屠宰场肉鸡中存在着较高的ALV-J感染。
In order to understand the health status of broilers to provide a basis for their risk assessment of food safety, 300 liver, 200 spleen and 100 glandular stomachs were collected from a broiler slaughterhouse in Beijing. HE staining was performed on the tissues Neutrophil histochemistry was used to detect the gp85 specific antigen of avian leukosis virus J subgroup (ALV-J) in liver tissue. ALV RNA in liver tissue was detected by PCR. The results showed that the histopathological examination showed that the detection rate of mass of marrow-like cells in the liver was 43% and lymphocytic infiltration lesions accounted for 75%. A small amount of lymphocytes were necrotic and emptying in spleen, and 52% 25% of gastric mucosal lymphocyte infiltration. Immunohistochemistry showed that the positive rate of ALV-J gp85 antigen in liver samples was 61.7%, the positive rate in spleen was 66.5%, and the positive rate in stomach and stomach samples was 52.6%. The positive rate of ALV-J gp85-specific PCR product was 30.5%. GenBank comparison showed that the homology with each strain ALV-J was 85% -90%. The results show that there is a high ALV-J infection in this slaughterhouse.