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目的运用cDNA微阵列技术对苯中毒肿瘤相关基因表达谱进行分析研究。方法对7例接苯中毒工人及7例正常对照者全血中淋巴细胞分别进行RNA抽提,纯化后的mRNA配对进行逆转录制备杂交探针,利用7张含有2780个cDNA克隆的微列阵肿瘤相关基因芯片进行杂交,杂交信号用扫描仪进行扫描,然后将图像转化为基于荧光强度的数字信号,随后进行数据分析和处理。结果在7张肿瘤相关基因中共发现特异性差异表达基因44个,其中GRO1、TGFBR3、LYN等16个基因表达上调,FOSB、DJ1、MCT1等28个基因表达下调。结论苯暴露患者肿瘤相关基因的表达异常,提示其可能为致白血病的关键基因,在苯致白血病中起着重要的作用;采用基因芯片检测技术有利于全面揭示苯中毒肿瘤相关基因表达模式,快速高效地发现新的研究目标和基因治疗途径。
OBJECTIVE: To analyze the expression profiles of tumor associated genes in benzene poisoning by cDNA microarray. Methods The whole blood lymphocytes from 7 benzene-exposed workers and 7 normal controls were extracted by RNA. The purified mRNA was reverse-transcribed to prepare hybridization probes. Seven microarrays containing 2780 cDNA clones The tumor-associated gene chips are hybridized, the hybridization signal is scanned by a scanner, and then the image is converted into a digital signal based on fluorescence intensity, followed by data analysis and processing. RESULTS: Totally 44 differentially expressed genes were found in 7 tumor-associated genes. Among them, 16 genes such as GRO1, TGFBR3 and LYN were up-regulated, and 28 genes such as FOSB, DJ1 and MCT1 were down-regulated. Conclusion Abnormal expression of tumor-related genes in benzene exposure patients suggests that it may be a key gene for leukemia and plays an important role in the treatment of benzene-induced leukemia. Using gene chip detection technology is conducive to revealing the expression patterns of tumor-associated genes in benzene poisoning Discover new research goals and gene therapy pathways efficiently.