Glycogen synthase kinase-3β positively regulates the proliferation of human ovarian cancer cells

来源 :Cell Research | 被引量 : 0次 | 上传用户:jinmeng79
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Although glycogen synthase kinase-3(GSK-3)might act as a tumor suppressor since its inhibition is expected tomimic the activation of Wnt-signaling pathway,GSK-3β may contribute to NF-κ3 activation in cancer cells leading toincreased cancer cell proliferation and survival.Here we report that GSK-3β activity was involved in the proliferationof human ovarian cancer cell both in vitro and in vivo.Inhibition of GSK-3 activity by pharmacological inhibitors sup-pressed proliferation of the ovarian cancer cells.Overexpressing constitutively active form of GSK-3β induced entryinto the S phase,increased cyclin D1 expression and facilitated the proliferation of ovarian cancer cells.Furthermore,GSK-3 inhibition prevented the formation of the tumor in nude mice generated by the inoculation of human ovariancancer cells.Our findings thus suggest that GSK-3β activity is important for the proliferation of ovarian cancer cells,implicating this kinase as a potential therapeutic target in ovarian cancer. Although GSK-3β might act as a tumor suppressor since its inhibition is expected tomimic the activation of Wnt-signaling pathway, GSK-3β may contribute to NF-κ3 activation in cancer cells leading to invasive cancer cell proliferation and survival. Here we report that GSK-3β activity was involved in the proliferation of human ovarian cancer cell both in vitro and in vivo. Inhibition of GSK-3 activity by pharmacological inhibitors sup-pressed proliferation of the ovarian cancer cells. Overexpressing constitutively active form of GSK-3β induced entryinto the S phase, increased cyclin D1 expression and facilitated the proliferation of ovarian cancer cells. Future Advanced, GSK-3 inhibition prevented the formation of the tumor in nude mice generated by the inoculation of human ovarian cancer cells. My findings therefore suggest that GSK-3β activity is important for the proliferation of ovarian cancer cells, implicating this kinase as a potential therapeutic target in ovarian can cer.
其他文献
油井井筒液体的热量损失取决于井筒附近地层温度分布,有关井筒附近地层温度分布的计算,雷米(Ramey)曾于1962年建立了相应的计算模型,但模型中无穷小井筒半径的假设在某些情况
经热处理分会学术工作委员会委员的提名及打分两轮评选 ,《金属热处理》及《金属热处理学报》中的以下两篇论文评为 1999年度优秀论文 :黄建洪 ,刘东雨 .低合金刃具钢的姜块
1我们,就像难得一遇的宿敌没有想到,刚刚回家乡的我,就和奶奶有了矛盾。我的母亲是南方人,父亲是北方人,他们结婚后一直都在南方发展,以致听惯当地方言的我,回到北方老家后,
岩心气体渗透率的测定结果表明,岩心的气体渗透率是围压的函数。随围压的增加,气体渗透率逐渐变小。围压对低渗透率岩心的影响比高渗透率岩心大得多。同时还表明,岩心的气体
我的家在东北,还没到松花江上,距祖国的心脏约1000km,虽然算不得肢端末梢,但至少偏离了躯干部分。每年春节,我都老老实实地随着春运大潮挤火车回家,今年却活泛起了小心思,因
一个题为“大兴——首都新世纪发展空间”的会议日前在北京大兴区召开。会议提出,我国城郊经济发展迫切需要“二次创业”。 中国城郊经济研究会会长包永江认为,我国的城郊经
作者最近对山西省河津县境内的兴国寺镇风塔进行了初步考察,发现这座始建于宋的佛塔具有一种奇特的声学效应,即所谓“鸟鸣现象”.在实地考察的基础上,本文对该塔声学效应的规
介绍了质子激发X射线荧光法测定样品中微量元素的原理和实验方法;给出了胃癌高、低发环境区白菜中微量元素的含量,提示了高含量Cr,Fe,Cu及低Se可能与胃癌高发有关,此种测试方法为探讨胃癌高
目的通过建立与肝组织生理和病理硬度相当的体外培养模型,探讨基底硬度和TGF-β1协同作用对肝细胞表型变化的影响。方法采用免疫荧光显微观测技术及Western Blotting等试验方
目的通过插入失活α毒素基因获得产气荚膜梭菌(Clostridium perfringens)突变体。方法利用MobilegroupⅡintron的自我剪切作用,对本室保藏的1株野生型C.perfringens的α基因p