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目的观察整合素相连激酶(ILK)和基质金属蛋白酶9(MMP-9)在人非小细胞肺癌(NSCLC)中的表达,初步探讨其与NSCLC预后的关系。方法选取2002年1月至2004年1月于南京医科大学附属第一医院手术切除的75例NSCLC患者标本,采用免疫组织化学方法检测NSCLC组织中ILK和MMP-9的表达水平,根据所得积分光密度(IOD)的中位数将75例患者分为ILK高表达组和低表达组,MMP-9高表达组和低表达组。采用Log-rank检验比较各临床病理特征及ILK、MMP-9表达对生存的影响,并对ILK和MMP-9的表达进行相关性分析。结果 ILK和MMP-9在NSCLC组织中均有表达,相关性分析显示:在本组75例患者中,ILK与MMP-9的表达呈正相关(r=0.79,P<0.05)。术后随访75例,随访至2009年1月31日时,中位生存时间30个月。ILK与MMP-9低表达组患者生存时间显著高于ILK和MMP-9高表达组(χ2=15.067,14.031,P<0.05);患者性别、年龄、吸烟史和病理类型对其生存期影响差异均无统计学意义(χ2=0.450,0.078,1.460,1.623,P>0.05);而肿瘤直径、淋巴结转移、TNM分期ILK及MMP-9表达对患者生存期影响差异均有统计学意义(2χ=3.936,15.169,20.529,15.067,14.301,P<0.05)。结论 ILK和MMP-9的表达水平影响NSCLC患者的预后,MMP-9可能通过ILK途径促进肿瘤细胞浸润、转移。因此,ILK和MMP-9的表达检测对评估NSCLC患者预后可能具有重要作用。
Objective To investigate the expression of ILK and MMP-9 in human non-small cell lung cancer (NSCLC) and to investigate its relationship with the prognosis of NSCLC. Methods Seventy-five patients with NSCLC surgically removed from the First Affiliated Hospital of Nanjing Medical University from January 2002 to January 2004 were enrolled in this study. The expression of ILK and MMP-9 in NSCLC tissues was detected by immunohistochemical method. The median of density (IOD) 75 patients were divided into high ILK expression group and low expression group, MMP-9 high expression group and low expression group. Log-rank test was used to compare the clinicopathological features and ILK, MMP-9 expression on the survival, and ILK and MMP-9 expression correlation analysis. Results ILK and MMP-9 were all expressed in NSCLC tissues. Correlation analysis showed that there was a positive correlation between ILK and MMP-9 expression in the 75 patients (r = 0.79, P <0.05). Follow-up 75 cases were followed up to January 31, 2009, the median survival time of 30 months. The survival time of patients with low expression of ILK and MMP-9 was significantly higher than that of patients with high expression of ILK and MMP-9 (χ2 = 15.067,14.031, P <0.05). The gender, age, smoking history and pathological type had different effects on survival (Χ2 = 0.450,0.078,1.460,1.623, P> 0.05). The differences of tumor diameter, lymph node metastasis, TNM stage ILK and MMP-9 expression in patients’ survival were statistically significant (2χ = 15. 369, 15. 529, 15.067, 14.301, P <0.05). Conclusion The expression levels of ILK and MMP-9 affect the prognosis of patients with NSCLC. MMP-9 may promote tumor cell infiltration and metastasis through ILK pathway. Therefore, the detection of ILK and MMP-9 expression may play an important role in assessing the prognosis of patients with NSCLC.