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应用体外方法确定恶性疟原虫对氯喹的敏感度是根据培养管内不同浓度的氯喹对疟原虫从小滋养体发育到裂殖体的抑制作用而做出判断的。方法是在每个培养管中加入一滴50%的无菌葡萄糖液,并分别加入0.25、0.5、0.75、1.0、1.5、2.5和3毫微克分子量的氯喹(毫微克分子=10~(-9)克分子)。另设不加药的对照管,在每管内加入1毫升脱去纤维蛋白的血,在38.5～40℃中培养20～30小时,于培养20小时后,用火焰消毒过的定量吸管从对照管中吸取一滴血制成薄血膜,观察疟原
In vitro method to determine the sensitivity of Plasmodium falciparum to chloroquine is based on the different concentrations of chloroquine in the culture tube on the inhibition of Plasmodium from trophozoite development to schizonts. One drop of 50% sterile glucose solution was added to each culture tube and 0.25, 0.5, 0.75, 1.0, 1.5, 2.5, and 3 nanograms of chloroquine (nanogram = 10-9) Mole). Another set without drug control tube, in each tube by adding 1 ml of defibrinated blood, cultured at 38.5 ~ 40 ℃ for 20 to 30 hours after the culture for 20 hours, flame-sterilized quantitative pipette from the control tube Draw a drop of blood into a thin blood film, observe the malaria