目的研究龙葵碱对乳腺癌MCF-7细胞微管系统的影响。方法 MTT法检测龙葵碱对人乳腺癌MCF-7细胞增殖的抑制作用,流式细胞仪分析龙葵碱对MCF-7细胞周期的影响以及细胞内α-微管蛋白及微管相关蛋白(MAP-2)的变化。结果龙葵碱对MCF-7细胞的IC50为22.08μg/mL,能够将MCF-7细胞阻滞于S期;能够增加MCF-7细胞内α-微管蛋白和MAP-2的量。结论龙葵碱通过升高MCF-7细胞内的微管蛋白及MAP-2的表达,将MCF-7细胞阻滞于S期,从而抑制乳腺癌MCF-7细胞的生长。 Objective To study the effect of solanine on the microtubule system of breast cancer MCF-7 cells. Methods The inhibitory effect of solanine on the proliferation of human breast cancer MCF-7 cells was detected by MTT assay. The effect of solanine on the cell cycle of MCF-7 cells was analyzed by flow cytometry. The effects of alanine-base on the proliferation of MCF- MAP-2) changes. Results Solanine had an IC50 of 22.08 μg / mL on MCF-7 cells, which could arrest MCF-7 cells in S phase and increase the amount of α-tubulin and MAP-2 in MCF-7 cells. Conclusion Solanine inhibits the growth of MCF-7 breast cancer cells by increasing the expression of tubulin and MAP-2 in MCF-7 cells and arresting MCF-7 cells in S phase.